whatisthedifferencebetweenfluorescenceandluminescence.docVIP

MCB 730: Microscopy Lab Class: Weds 4/21 1:10 – 5 PM in Porter 305 Instructor: Janet Duerr, duerr@, 597-1921 YOU ARE REQUIRED TO BRING THE COMPLETED PRE-QUIZ TO CLASS Read the included material on microscopy and C. elegans BEFORE class so that are prepared to observe and photograph GFP-expressing C. elegans. Learning Objectives: Gain an understanding of the features of transmitted light microscopes. Review and remember enough optics to understand how light is altered as it interacts with lens, diaphragms, and the sample. Use this understanding to learn how to adjust the features in any standard light microscope to enhance visualization of specific samples. Gain an understanding of how a camera and computer capture and save images. Determine the effects of changing brightness, contrast, gamma, offset, exposure time, aperture, etc. Learn how to manipulate the settings to enhance visualization of a specific type of sample. Review and remember enough optics to understand how fluorescence works. Gain an understanding of the features of fluorescent light microscopes. Review and remember enough optics to understand how light is altered as it interacts with lens, FILTERS, diaphragms and the sample. Use this understanding to learn how to adjust the features in a fluorescence microscope to enhance visualization of specific samples. Use this understanding to learn how to adjust the camera, computer, and microscope to record data without damage to the samples. Learn enough about fluorescent light microscopes to improve your ability to design experiments using fluorescent tags. The Microscope – Introduction Adapted from: Heidcamp /~cellab A compound microscope is composed of two elements; a primary magnifying lens and a secondary lens system, similar to a telescope. Light is caused to pass through an object and is then focused by the primary and secondary lens. If the beam of light is replaced by an electron beam, the microscope becomes a transmission ele