测量脉冲率lumbriculusvariegatus（ measuringthe pulse rateof lumbriculusvariegatus）.docVIP

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测量脉冲率lumbriculusvariegatus（ measuringthe pulse rateof lumbriculusvariegatus）.doc

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What Makes a Worm’s Heart Race? Part 1 Student Lab Guidelines Worksheet Name: Date: Hour: Purpose: To explore the impact of treatments on the pulse rate of the worm Lumbriculus variegatus while refreshing and refining your understanding of experimental design with a focus on recognizing and minimizing experimental bias. Objectives: Upon completion of this activity, you should be able to: Count pulse rate in blackworms (Lumbriculus variegatus). Demonstrate the ability to design and perform an experiment using the strong inference model and multiple hypotheses with attention to the minimization of experimental bias. Demonstrate an understanding of graphing and the use of simple statistical data analysis to evaluate the impact of an experimental treatment. Explain the relationship between dependent and independent variables (in this particular case – the pulse rate of the blackworms and the treatment with a stimulant/depressant/toxin). Technique Practice and Gathering of Baseline Data (Day 1): Read and complete Use of Animals in the Classroom (Addendum A at the back of this packet). Have your instructor initial your form after you read and sign it. For every 2 students get: Petri dish 2-3 blackworms in spring water (from the class container) and place these in your Petri dish ? piece of plastic capillary tube Thin stem pipette Compound microscope or dissecting/stereoscope Stopwatch Draw one worm into your thin stem pipette. Squirt the worm gently into the capillary tube. Place the worm in the tube on the stage of your compound microscope. Focus on low power (red objective) if using compound microscope, high if using stereoscope Inspect your worm by moving (including rolling) the capillary tube – find the head and tail and the dorsal (back) and ventral (front or belly) sides. Answer the following questions as you explore your worm (record your answers here): What color is your blood? _____________ What color is the blackworm’s blood? ______________