HRPconjugatedtoanti-M13Monoclonal..docVIP

GE Healthcare HRP/Anti-M13 Monoclonal Conjugate Horseradish Peroxidase conjugated to anti-M13 Monoclonal Antibody with Mouse ascites fluid Product Specification Sheet Code: 27-9421-01 Warning For research use only. Not recommended?or?intended for diagnosis of disease in humans?or?animals. Do not use internally?or?externally in humans?or?animals. Storage Store at -15°C to -30°C. Components HRP/anti-M13 Monoclonal Conjugate consists of horseradish Peroxidase conjugated to mouse anti-M13 monoclonal antibody. The antibody purified from BALB/c mouse ascites fluid, reacts specifically with the bacteriophage M13 major coat protein product of gene VIII (gp8). The conjugate is supplied in 0.8 ml of 50% glycerol/0.5 x PBS for standard usage at a 1:5000 dilution (200 μl/microplate well). This provides sufficient HRP/anti-M13 Monoclonal Conjugate for 200 microplate (96 well) detection assays using the protocol supplied. Other materials required ? 1 x PBS To prepare 1 x PBS, add the following to a 1 litre flask. NaCl 8 g KCl 0.2 g Na2HPO4 1.44 g KH2PO4 0.24 g Distilled water to 1 litre. Adjust pH to 7.4 with HCL. Sterilization is not necessary. ? 1 x Blocking buffer (2% non-fat dry milk in 1 x PBS). Dissolve 20 g of non-fat dry milk in 1 litre of 1 x PBS. Note: Prepare fresh daily. ? Wash buffer (1 x PBS with 0.05% Tween?20). Add 0.5 ml Tween 20 to 1 litre of 1 x PBS, mix and use. Note: Prepare fresh daily. ? 0.05 M Citric Acid Dissolve 10.507 g of Citrate monohydrate (mw 210.14) in 1 litre of distilled water. Adjust the pH to 4.0 with 10 N NaOH (~6 ml of base will be required). Filter sterilize through a 0.22 μm filter. ? 1 x ABTS Stock Solution Add 0.22 mg of ABTS to every 1 ml of 0.05 M Citric acid, pH 4.0. Prepare fresh?or?sterilize and store at 2–8°C until required. ? ABTS Substrate Solution Immediately before use add 36 μl of a 30% H2O2 solution to 21 ml of 1 ATBS stock solution for each 96-well microplate. Safety warnings and precautions All chemicals should be considered