研究颜面赝复体粘接剂的体外细胞毒性.docVIP

研究颜面赝复体粘接剂的体外细胞毒性【摘要】 目的： 利用体外细胞培养技术, 对有机硅改性聚丙烯酸酯类颜面赝复体粘接剂（FPSA）的细胞毒性进行评价. 方法： 采用MTT法评价FPSA的细胞毒性. 利用体外培养的小鼠肺成纤维细胞(L在培养液中分别添加500，750 mL/L FPSA浸提液,于加样后2，4，7 d用MTT法显色, 用多道扫描酶标仪测A490 nm值,计算相对增殖率,对FPSA的细胞毒性进行评价. 结果： 随着时间推移,不同浓度的FPSA浸提液对L细胞增殖均无明显作用(P0.05), 各浓度FPSA浸提液加样后2，4，7 d之间差异无统计学意义(P0.05),提示L细胞增殖与浸提液浓度无明显相关性,FPSA的细胞毒性分级为1级. 结论: FPSA无明显细胞毒性, 具有良好细胞相容性, 是一种有发展前景的颜面赝复体粘接材料. 【关键词】 颜面部缺损；赝复体粘接剂；细胞毒性；MTT法 　　0引言 　　颜面赝复体的固位中组织倒凹和机械固位多用作辅助方式,种植体固位虽然是颜面赝复体首选的固位方式［1］,但由于多种原因使患者不能接受种植手术时, 就需依赖粘接固位. 传统的赝复体粘接剂中,聚丙烯酸酯类粘接剂价廉,粘接强度尚可,但皮肤上的粘接剂残留较难去除［2］；有机硅类粘接剂粘接强度高,皮肤残留少,但含有机溶剂具有潜在刺激性［3］. 我们通过乳液聚合的方法合成有机硅改性聚丙烯酸酯类的颜面赝复体粘接剂（facial prosthesis）,并参照国际标准化组织发布的医疗器械生物学评价标准,进行体外细胞毒性试验［4］,将小鼠肺成纤维细胞(L County economic work conference leadership speech:The main task of the twenty-six session of the six plenary meeting of the county the county economic work conference this time, by the partys eighteen and eighteen Third Plenary Session of the spirit as a guide, carry out and implement the central and provincial economic work conference on rural work conference and the three session of the seven plenary session of the party to expand the spirit of the meeting, the total summary of last year the work, analysis of the current situation, the deployment of this work, further strengthening the development of consensus, to mobilize the whole county county, deepening reform and innovation, to work against poverty, accelerate the transformation of leapfrog, promote scientific development, struggling to write a new harmonious beautiful mountains and show a prosperous civilization to build a new building. The following chapter * *, according to the county Discussion of the views of the Standing Committee, I focus on four aspects of the problem:* * * years, the county to seriously implement the central decision-making arrangements and real, in accordance with the high position, as a typical tree, catch catch up with speed for a breakthrough, five in one overall grasp to raise human resources to pro