广东省动物源沙门菌PMQR和ESBLs基因流行分布调查..docVIP

广东省动物源沙门菌PMQR和ESBLs基因流行分布调查 摘要：【目的】检测分离自广东省散养型养殖场的动物源沙门菌中质粒介导喹诺酮类耐药基因(PMQR)和β-内酰胺类基因（ESBLs）的流行情况。【方法】琼脂平板稀释法对阳性菌株进行16种抗菌药物的最小抑菌浓度（MIC）测定；采用PCR方法检测PMQR(qnr、qepA、oqxAB、aac(6’)-Ib-cr)和ESBLs(CTX-M、TEM、SHV、OXA、PER、VEB、GES、CMY-2)基因。【结果】72株沙门菌对16种兽医临床产用抗生素耐药率较高；qnrA 、qnrD、qnrS、aac（6′）-Ib-cr、oqxAB的检出率依次为6.94%、25%、5.56%、33.33%和6.39%，但未检测到qnrB、qnrC和qepA；aac（6′）-Ib-cr 的检出率较高，且与oqxAB和qnrD 常常同时存在；CTX-M(11.11%)、PER（1.39%）、CMY-2(9.72%)、TEM（56.94%）、OXA（69.44%），没有检测到VEB、GES、SHV。【结论】PMQR和ESBLs基因在广东省兽医临床传播广泛，且对临床常用抗菌药物耐药较为严重，药敏谱呈多样化，多重耐药株比例较高,需引起重视。 关键词：沙门菌；PMQR；ESBLs；耐药基因 Prevalence of ESBLs and PMQR Encoding Genes in Salmonella from animal origin in Guangdong Abstract：【Objective】The detected isolated from farm animals in Guangdong Province source of Salmonella in the plasmid-mediated quinolone resistance gene (PMQR) and β - lactams (ESBLs) gene prevalence。【Method】16 antimicrobial agents by agar dilution method of positive strains minimum inhibitory concentration (MIC) determination ；The PCR method to detect PMQR (qnr, qepA, oqxAB, aac (6 )-Ib-cr) and ESBLs (CTX - M, TEM , SHV , OXA , the PER , VEB , GES , CMY - 2 ) gene。【Results】72 strains of salmonella bacteria to 16 kinds of veterinary clinical antibiotic resistance rates higher；of qnrA , qnrD the qnrS , aac ( 6 ) - Ib - cr , oqxAB detection rates were 6.94% , 25% , 5.56 % , 33.33% and 6.39%，But not detected qnrB, qnrC , and qepA；AAC (6 ′) -Ib-cr detection rate is higher, and the oqxAB and qnrD often exist at the same time; CTX-M(11.11%)、PER（1.39%）、CMY-2(9.72%)、TEM（56.94%）、OXA（69.44%）; Not detected by VEB, GES, SHV。【Conclusion】PMQR and ESBLs gene is widely spread veterinary clinic in Guangdong Province；And for clinical commonly used antimicrobial drug resistance is relatively serious ，Drug susceptibility spectrum are diversified，A higher proportion of multiple antibiotic resistant strains，Need to bring to the attention。 Keywords： salmonella; PMQR; ESBLs; Resistance gene 引言： 自从喹诺酮类抗生素被应用于临床以后，耐药菌株便随之出现并日益严重[1] 。1998年首次在