毕业论文纳米硫化铅对雄性大鼠肝肾组织氧化损伤功能描述.doc

学 号：200895110131 毕业论文 GRADUATE THESIS 设计题目：纳米硫化铅对雄性大鼠肝肾组织氧化损伤功能 的影响 摘 要 目的：通过建立纳米硫化铅染毒模型，探讨PbS NPs对大鼠肝肾组织氧化损伤功能的影响，为深入阐明PbS NPs对肝肾的毒性机制，寻找有效干预措施提供依据。 方法：SPF级雄性大鼠50只，随机分为对照组（超纯水）；低剂量组（25mg/kg PbS NPs）、中剂量组（50mg/kg PbS NPs）、高剂量组（100mg/kg PbS NPs）；微米组（100mg/kg PbS），每组10只动物。每天灌胃染毒一次，每周五天，连续染毒6周后，进行各指标的测定：采用试剂盒测定肝肾组织抗氧化酶活力（SOD、GSH-Px、CAT）及MDA含量；采用H-E染色对肝肾组织进行病理形态学检测。 结果：PbS NPs染毒组大鼠肝肾组织SOD、GSH-Px、CAT的活力较对照组明显下降（Ｐ0.05），MDA含量较对照组上升（Ｐ0.05）。相同剂量下，PbS NPs组SOD、GSH-Px、CAT的活力均低于微米组，MDA含量高于微米组。 结论：PbS NPs染毒可致大鼠肝肾组织发生氧化损伤，而不同粒径比较，PbS NPs作用甚于微米PbS。 关键词 PbS NPs；SOD；CAT；MDA；氧化损伤 Abstract Objectives：To investigate the toxicity of oxidative damage induced by PbS NPs in rat liver and kidney by the establishment of PbS NPs exposure model, elucidate the mechanisms the liver and kidney toxicity of PbS NPs, and to provide evidences for effective interventions. Methods：50 SPF grade male rats were randomly divided into control group (ultrapure water), low dose group (25mg/kg PbS NPs), middle dose group (50mg/kg PbS NPs), high-dose group (100mg/kg PbS NPs) and micron group (100mg/kg PbS), each group with 10 rats. Exposure by gavage once a day, five days a week, continued exposure for 6 weeks. The determination of liver and kidney tissues using antioxidant enzymes (SOD, GSH-Px, CAT) and MDA kits and using HE staining to test liver and kidney tissues pathological changes. Results：The SOD, GSH-Px and CAT vigors in liver and kidney tissues of PbS NPs groups decreased significantly than the control group (P 0.05), but MDA contents increased significantly (P 0.05). Wiht same concentration, compared with the micron group, SOD, GSH-Px and CAT activities in PbS NPs groups were lower but MDA content were increased. Conclusions：PbS NPs exposure can cause oxidative damages in liver and kidney tissues of rats. Comparison of different sizes, the effects of PbS NPs stronger than micron PbS. Keywords PbS NPs; SOD; CAT; MDA; Oxidative damage 目 录 毕业论文 i GRADUATE THES