三七超微粉的质量标准研究.docVIP

三七超微粉的质量标准研究 【摘要】nbsp; 目的建立三七超微粉的质量标准。方法采用薄层色谱法鉴别三七；使用激光粒度检测仪对粒径进行检测；HPLC 法测定三七超微粉中三七皂苷R1，人参皂苷Rg1，人参皂苷Rb1含量。结果薄层图谱斑点清晰，可鉴别出与三七的对应斑点；三七超微粉的中位径在15 μm以下；三七皂苷R1 在0.535～3.210 μg 范围内线性关系良好( r =1.000) ,平均回收率103.08% ,RSD = 2.80%；人参皂苷Rg1 在2.225～13.350 μg 范围内线性关系良好(r = 1.000) ,平均回收率100. 33% , RSD=2.29 %；人参皂苷Rb1在2.205～13.230 μg范围内线性关系良好(r = 1.000),平均回收率101.00%, RSD = 1.43 %。结论所建立的方法简便、准确、重复性好,可用于三七超微粉的质量控制 【关键词】nbsp; 三七超微粉 粒径 高效液相 薄层色谱 质量标准 　　Abstract:ObjectiveTo develop a quality standard for super micropowdernbsp; of notoginseng.Methods Qualitative analysis ofnbsp; micropowder ofnbsp; notoginseng was carried out by TLC. The particle diameter was detected withnbsp; laser scattering particle size distribution anaslyzer. An RP - HPLC method was used for the content determination of the Notoginsensode R1, ginsenoside Rg1, ginsenoside Rb1. ResultsThe chromatographic spots of micropowder ofnbsp; notoginsengnbsp; were identified without the interference of negative control. Micropowder of particle average diameter was below 15μm. Notoginsensode R1 had a good linearity in the range of 0.535～3.210 μg (r = 1.000) ,and the average recovery was 103.08%nbsp; with RSD ofnbsp; 2.80%. Ginsenoside Rg1 had a good linearity in the range of 2.225～13.350 μg (r=1.000) ,and the average recovery was 100.33 % with RSD of 2.29 %. Ginsenoside Rb1 had a good linearity in the range of 2.205～13.230 μg ( r= 1.000) ,and the average recovery was 101.00% with RSD of 1.43%. ConclusionThis method isnbsp; simple ,accurate and repeatable. It can be used to determine ginsenoside Rg1，ginsenoside Rb1and notoginsensode R1 in micropowder ofnbsp; Notoginseng. 　　Key words:Micropowder ofnbsp; Notoginseng;nbsp; Particle diameter;nbsp; HPLC;nbsp; TLC;nbsp; Quality standard nbsp;nbsp;nbsp; 　　三七(Radix Notoginseng)为常见的贵重药材，目前市场上的三七超微粉已经较为普遍，但对三七超微粉的质量标准研究的文献报道较少，本课题采用薄层定性鉴别，使用激光粒度检测仪对三七超微粉的粒径进行检测，并在参考文献［1］的基础上，同时测定三七超微粉产品中三七皂苷R1，人参皂苷Rg1和人参皂苷Rb1的含量。方法简便、准确、重复性好，可用于三七超微粉的质量控制。 　　1nbsp; 仪器与试药 n