中华补血草根提取物抗四氯化碳急性肝损伤的实验研究.docVIP

中华补血草根提取物抗四氯化碳急性肝损伤的实验研究 nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp; 作者：汤新慧 高静 陈瑾 徐力致 【摘要】nbsp; 目的研究中华补血草根提取物（the Limonium sinense Ktze root extract, LSE）对小鼠急性肝损伤的防护作用及药理机制。方法建立小鼠四氯化碳（CCl4）肝损伤模型，检测LSE对血清天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）活性的影响；观察肝细胞超微结构的变化；测定肝线粒体膜电位以及线粒体对高钙诱发肿胀的敏感性的变化。结果一定剂量（100～400 mg/kg）LSE能有效抑制CCl4引起的小鼠血清AST及ALT活性的显著上升；明显减轻肝损伤引起的小鼠肝脏线粒体形态结构的破坏；抑制线粒体膜电位的降低以及线粒体对外加高钙诱发肿胀敏感性的下降。结论 LSE对CCl4诱导的急性肝损伤具显著的防护作用，其机理可能与保护肝脏线粒体有关。 【关键词】nbsp; 中华补血草 四氯化碳 肝损伤 线粒体 　　Abstract:ObjectiveTo study the hepatoprotective effect of Limonium sinense (Girard) Ktze root extract (LSE) and the possible mechanisms underlying its protection on acute liver injury induced by carbon tetrachloride (CCl4). Methods CCl4-induced liver injury model was used to evaluate the effect of LSE on the activities of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in mice. Ultrastructure of liver was observed and liver mitochondrial membrane potential and mitochondrial swelling were measured following CCl4 injection without or with LSE. ResultsWith oral pretreatment of 100 mg/kg, 200 mg/kg or 400 mg/kg LSE, the increase in serum AST and ALT activities induced by CCl4 were obviously reversed and significant morphological changes in liver mitochondria were remarkably lessened. In addition, the decrease in mitochondrial membrane potential and sensitivity of mitochondrial swelling to the exotic Ca2+ stimulation induced by CCl4 were also prevented by LSE. Conclusion LSE has hepatoprotective activity and the mechanisms underlying its protective effect may be related to its protection on liver mitochondria. 　　Key words:Limonium sinense (Girard) Ktze;nbsp;nbsp; Carbon tetrachloride;nbsp;nbsp; Liver damage;nbsp;nbsp; Mitochondria nbsp;nbsp;nbsp; 　　中华补血草Limonium sinense (Girard) Kuntz 系白丹花科补血草属多年生泌盐草本植物，喜生于沿海盐渍化的低洼湿地上，广泛分布于我国东北、华北及陕、甘、宁和苏北等地区。除具有较高观赏价值、环境保护等作用外，具有一定药用价值。成分分析表明，该植物化学成分十分丰富，含有多糖、鞣质、萜类、黄酮类，以及氨基酸、矿物质等［1～2］。据记载，该植