乳腺癌组织中pcna、bcl2与p53基因蛋白表达.docVIP

乳腺癌组织中PCNA、bcl2与p53基因蛋白表达 nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp; 作者：宾晓农　谭敏　何青莲　赵虹　刘鹏熙nbsp;nbsp; 【摘要】nbsp; 　　［目的］ 探讨乳腺癌、癌旁和正常乳腺组织中PCNA、bcl-2与p53基因蛋白表达的差异及其在乳腺癌变中的意义。［方法］ 用免疫组化法检测46例乳腺癌、癌旁组织和33例正常乳腺组织中PCNA、bcl-2与p53基因蛋白表达。［结果］ ①PCNA指数：乳腺癌与癌旁组织差异无显著性；乳腺癌和正常乳腺组织(46.03±8.76与32.13±6.78)，乳腺癌旁和正常乳腺组织(42.90±7.92与32.13±6.78)差异均有显著性(Plt;0.01)。②bcl-2基因蛋白表达分值：乳腺癌、癌旁和正常乳腺组织分别为：0.62±0.47，0.91±0.58，1.56±0.84；分值大小顺序呈表达上升趋势。③p53基因蛋白表达分值：乳腺癌、癌旁组织和正常乳腺组织分别为：2.21±0.96，1.86±0.87，0.87±0.57；分值大小顺序表达呈下降趋势。④在乳腺癌组织PCNA指数与bcl-2呈中度负相关(rs=-0.537)，与p53呈中度正相关(rs=0.628)，bcl-2与p53呈中度负相关(rs=-0.476)。［结论］ 乳腺癌的发生、发展过程中PCNA和p53分值增大，bcl-2分值变小，预示组织恶性程度可能增加，对判定乳腺组织的良、恶性及预后具有重要临床价值。 【关键词】nbsp; 乳腺肿瘤　增殖细胞核抗原　bcl-2　p53 　　 Expression of PCNA, bcl-2 and p53 Proteins in Breast Cancer nbsp;nbsp;nbsp;nbsp;nbsp;nbsp; Abstract: ［Purpose］ To explore difference of expression of PCNA, bcl-2 and p53 proteins among breast carcinoma, para-carcinoma tissue and normal breast tissue and its value in development of breast carcinoma. ［Method］ PCNA, bcl-2 and p53 proteins in carcinoma tissue and para-carcinoma tissue in 46 patients with breast cancer and in 33 samples of normal breast tissue were detected by immunohistochemical S-P method. ［Results］ ①PCNA-PI showed no significant difference between breast carcinoma and para-carcinoma tissue (Pgt;0.05), but significant differences were found between breast carcinoma and the normal breast tissue (46.03±8.76 vs. 32.13±6.78), and between para-carcinoma tissue and the normal breast tissue (42.90±7.92 vs. 32.13±6.78, Plt;0.01). ②The expression score of bcl-2 was 0.62±0.47 in breast carcinoma, 0.91±0.58 in para-carcinoma tissue and 1.56±0.84 in normal breast tissue, with a increasing trend from cancer to normal tissue. ③The expression score of p53 was 2.21±0.96, 1.86±0.87 and 0.87±0.57 in breast carcinoma tissue, para-carcinoma tissue and the normal breast tissue, with a decreasing trend from cancer to normal tissue