人参二醇促骨髓cd34+细胞增殖和分化的研究.docVIP

人参二醇促骨髓CD34+细胞增殖和分化的研究 nbsp;nbsp;nbsp;nbsp;nbsp; 作者：方桂伦 高瑞兰 林筱洁 金锦梅 【摘要】nbsp; 本研究探讨人参二醇 (PDS) 对正常人骨髓CD34+细胞的促进增殖和诱导分化作用。用吸附单克隆抗体的免疫磁珠技术从正常人骨髓分离出CD34+ 细胞，采用琼脂半固体多向祖细胞集落（CFUMix）培养方法观察PDS对CD34+ 细胞的增殖作用；用液体培养使CD34+细胞增殖，并向红系、粒系定向生长，用流式细胞仪分析PDS诱导后细胞表面标记变化。结果显示：免疫磁珠分离CD34+ 细胞的获得率占骨髓有核细胞（MNC）的（1.0±0.15）%；活细胞比例占（95.6±1.2）%，CD34+ 细胞富集度达（86.8±2.8）%。 中浓度PDS （25 mg/L）能够有效地促进CD34+ 细胞增殖，生成CFUMix集落，提高率为（56.3±3.5）%，与对照相比较有显著差异（plt;0.01）。液体培养生成粒系、红系细胞的数量明显增高，与对照相比差异有显著性，提高率分别为（35.6±3.2）%和（22.3±2.1）%，与对照相比差异有显著性（plt;0.01），且呈剂量依赖性。经PDS诱导14天后,细胞表面分化标记明显增高，CD33+ 细胞在PDS 50 mg/L时最高，CD71+ 细胞在25 mg/L时最高，GA+ 细胞在10 mg/L时最高，而CD15+ 细胞数量无明显变化。结论：PDS不但促进CD34+细胞增殖，且能诱导其定向分化，提示PDS具有类似造血生长因子和协同生长因子的效应。 【关键词】nbsp; 人参二醇 nbsp;nbsp;nbsp; Effects ofnbsp; Ginsengnbsp; Panaxadiol Saponin onnbsp; Proliferation and Differentiation of Human Bone Marrow CD34+ Cells nbsp;nbsp;nbsp; Abstractnbsp;nbsp;nbsp; The study was purposed to investigate the effects of the panaxadiol saponinnbsp; (PDS)nbsp; from Ginseng on proliferation and differentiation of human CD34+ cells from human bone marrow. Highly purified CD34+ cells were isolated from human bone marrow by using the Dynal CD34 Cell Selection System (Dynal, Norway). The cells were exposed to PDS at various concentrations in both agar semisolid culture of CFUMix and suspension culture of myeloid and erythroid cells in order to observe the effects of PDS on proliferation of CD34+ cells. The cells were marked with 4 kinds of monoclonal antibody in related with their differentiation toward to myeloid and erythroid lineages, then examined by flow cytometry (FACS) after beingnbsp; incubated with PDS for 14 days. The results showed that the number of CD34+ cells was 1.0?.15% out of marrow nuclear cells after being purified by Dynal beads system. Thenbsp; enrichment ofnbsp; CD34+ cellsnbsp; reached tonbsp; 86.8?.8 %. The best efficiency in promoting proliferation of CD34+ cells in vitro was obtained when the concentration of PDS was