人脐带静脉灌注液间充质干细胞的分离和鉴定.docVIP

人脐带静脉灌注液间充质干细胞的分离和鉴定 nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp;nbsp; 作者：刘晓丹，刘兵，李秀森，毛宁 【摘要】nbsp; 本研究旨在探讨人脐带静脉灌注液中是否存在间充质干细胞及其分离和扩增方法。从正常产妇分娩后的脐带静脉灌注液分离间充质干细胞，观察细胞形态并用流式细胞仪分析其细胞表型和细胞周期，在体外分别诱导其成骨、成脂肪和成软骨细胞分化。结果表明：脐带静脉灌注液来源的间充质干细胞为成纤维细胞样，高表达CD29、HLAABC、CD166、CD105、CD73、CD44；不表达造血和内皮标志（CD34、CD45、CD144和CD14）。它们可向骨、脂肪和软骨细胞分化，符合间充质干细胞的基本功能特征。结论：人脐带静脉灌注液存在间充质干细胞，能够在体外培养和扩增，可作为今后细胞治疗和组织工程的种子细胞。 【关键词】nbsp; 脐带静脉灌注液 nbsp;nbsp;nbsp;nbsp; Isolation and Identification of Mesenchymal Stem Cells from Perfusion of Human Umbilicalnbsp; Cord Vein nbsp;nbsp;nbsp;nbsp;nbsp; Abstractnbsp;nbsp;nbsp; This study was aimed to investigate whether mesenchymal stem cells (MSCs) existed in human umbilical cord vein and to establish the methods of isolation and expansion in vitro. The MSCs direved for perfusion of umbilical cord vein (UVMSCs) were collected after parturition of Healthy pregnant women. The morphology of MSCs and their differentiation potential into osteoblast, adipocyte and chondrocyte were observed the phenotype and cell cycle of MSCs were determined by using flow cytometry. The result showed that the mesenchymal stem cells separated from umbilical cordnbsp; vein gave rise to a population of adherent cells with a typical fibroblastlike morphology. Similarly to bone marrowderived MSCs,nbsp; they highly expressed CD29, HLAABC, CD166, CD105, CD73 and CD44, and were negative for any hematopoietic and endothelial markers (CD45, CD34, CD14 and CD144). Functionally, they could differentiate into the osteoblast, adipocyte and chondrocyte. It is concluded thatnbsp; MSCs exist in the human umbilical cord vein perfusion.nbsp; Their read amplification in vitro contribute tonbsp; clinical applications for cell therapy and tissue engineering. nbsp;nbsp;nbsp; Key wordsnbsp;nbsp;nbsp; mesenchymal stem cell; umbilicalnbsp; cord vein perfusion; cellnbsp; isolation; cell identification nbsp;nbsp;nbsp; 间充质干细胞（mensenchymal