何首乌蛋白质和蒽醌苷对d半乳糖衰老小鼠学习记忆及代谢产物的影响.docVIP

何首乌蛋白质和蒽醌苷对D半乳糖衰老小鼠学习记忆及代谢产物的影响 【摘要】nbsp; 　　【目的】观察何首乌蛋白质和蒽醌苷对D半乳糖所致衰老小鼠学习记忆与相关酶及代谢产物的影响。【方法】选用昆明种小鼠80只，随机分为8组：正常组，模型组，维生素E（VE）组（剂量为90mg·kg-1·d-1），何首乌蛋白质（PRPM）高、低剂量组（剂量分别为20、5mg·kg-1·d-1），何首乌蒽醌苷（AGRPM）高、低剂量组（剂量分别为28、7mg·kg-1·d-1），AGRPM与PRPM混合组（混合组，均取两者低剂量）；除正常组外，其他组均采用长期（7周）皮下注射D半乳糖复制衰老小鼠模型。采用三等分辐射式迷宫测试小鼠的学习速率，用一次性被动回避反应测试小鼠的记忆能力；采用紫外可见分光光度计测定小鼠脑组织超氧化物歧化酶（SOD）和单胺氧化酶（MAO）的活力及脂质过氧化产物丙二醛（MDA）的含量；采用荧光分光光度计测定肝组织中脂褐素（LF）的含量。【结果】高剂量PRPM组能显著改善衰老小鼠的记忆能力（P＜005），高剂量AGRPM组可显著改善衰老小鼠的学习速率和记忆能力（P＜005或P＜001）；PRPM组和AGRPM组均能显著增加衰老小鼠的肝系数、胸腺系数、脑系数和脾系数（P＜005或P＜001），降低衰老小鼠脑组织MAO的活力、脂质过氧化产物MDA的含量及肝组织中LF的含量（均P＜005或P＜001）；PRPM、AGRPM低剂量组及混合组均能显著增加衰老小鼠脑组织SOD的活力（P＜005或P＜001）。【结论】何首乌蛋白质和蒽醌苷具有一定的抗衰老及促进记忆能力的作用。 【关键词】nbsp; 何首乌/药理学；衰老/中药疗法；何首乌/分离与提纯；疾病模型，动物；小鼠 　　Abstract: ObjectiveTo observe the effects of protein and anthraquinones glycosides from Radix Polygoni Multiflori (RPM) on learning and memory as well as associated enzymes and metabolites in mice with Dgalactoseinduced aging. Methods Eighty Kunming mice were equally randomized into 8 groups: normal group, model group, vitamin E (90mg·kg-1·d-1) group, highand lowdose RPM protein (20 and5 mg·kg-1·d-1 respectively) groups, high and lowdose RPM anthraquinone glycoside (28 and 7mg·kg-1·d-1 respectively) groups, combination (lowdose RPM protein and lowdose RPM anthraquinone glycosides)group. Except the normal group,the mice in other groups received subcutaneous injection of Dgalactose to indue the aging model.The learning rate of mice was detected with trisection radiation maze, memory was evaluated by onetime passive avoidance response, cerebral superoxide dismustase (SOD) and monoamine oxidase (MAO) activities as well as malondialdehyde（MDA）content were detected with ultraviolet spectrophotometer, and hepatic lipofuscin (LF) content was examined with fluorescence spectrophotometer. Results Memory of aged mice was much improved in highdose RPM protein group (P＜005), and learning rate and memory