血吸蟲感染小鼠及家兔抗Sj23kDa膜蛋白大亲水肽段(Sj23HD)的抗体IgG应答模式及其免疫诊断价值.docVIP

血吸虫感染小鼠及家兔抗Sj23kDa膜蛋白大亲水肽段(Sj23HD)的抗体IgG应答模式及其免疫诊断价值 张伟§，王玠§余传信*宋丽君殷旭仁钱春艳许永良（）HSA和SEA检测132 [基金项目] 国家自然科学基金；江苏省自然科学基金（BK2008110）国家重大专项（2008Z；江苏省卫生厅科技项目（H20066）；YZ201030） ； [作者单位] 江苏省血吸虫病防治研究所[作者简介]，，。研究方向：王玠，女， 员。研究方向：血吸虫病分子免疫与诊断 通作者：chxnyu@163.comyangjianliang101@126.com; §为共同第一作者。 份血吸虫病人血清，阳性率分别为88.64%和97.73%；检测10份肺吸虫病人血清，交叉反应率为50%和90%；检测30份肝吸虫病人交叉反应为0%和16%；检测80份健康人血清，均无交叉反应。结论 本研究证明血吸虫感染小鼠、家兔血清中抗Sj23HD蛋白的抗体反应为抗原依赖的短寿抗体反应，检测参与这一特异性短寿抗体反应的抗体具有血吸虫病诊断与疗效考核潜能。与SEA相比，重组Sj23HD蛋白用于血吸虫病诊断具有较好的特异性，但对于来自肺吸虫流行区的患者仍需注意鉴别诊断。 [关键词] 日本血吸虫；23kDa膜蛋白大亲水肽段；短寿抗体反应；免疫诊断；疗效考核 The antibody response pattern of Sj23HD in mice and rabbits infected with Schistosoma japonicum and immunodiagnostic value ZHANG Wei§, WANG Jie§, YU Chuan-xin*, SONG Li-jun, YIN Xu-ren, QIAN Chun-yan, SHEN Shuang, JING Yi, KE Xue-dan, YAO Yuan, GAO Hong, XU Yong-liang, YANG Jing Department of Novel Biotechnology of Parasitic Disease Prevent and Control, Jiangsu Institute of Parasitic Diseases; Key Laboratory on Technology for Parasitic Disease Prevention and Control, Ministry of Health, Wuxi 214064, China * Corresponding author, §These authors contributed equally to this work. 【Abstract】 Objective To investigate the antibody response pattern of the large hydrophilic doman of 23 kDa membrane protein in mice and rabbits infected with Schistosoma japonicum, and observe the value of immunodiagnosis and evaluating chemotherapeutic effect of the recombinant fusion protein of Sj23HD-HSA for schistosomiasis japonica. Methods The sera of mice and rabbits infected with Schistosoma japonicum before infection, during infection and after cure with PZQ at different time were prepared. To observe the antibody response pattern of Sj23HD in mice and rabbits infected with Schistosoma japonicum and the potential of evaluating chemotherapeutic effect of schistosomiasis, the dynamics of antibody response against Sj23HD before infection, during infection and after cure with PZQ at different time were detected by