experiment membrane transport- departmentof zoology, u b c（实验膜运输- departmentof动物学、u b c）.doc

Experiment Membrane Transport Objectives ? Referring to energy, what two ways can substances enter a cell? What is active transport? What is passive transport? How is osmosis related to diffusion? How can we demonstrate active transport? How can we demonstrate Brownian movement? How can we demonstrate diffusion (2 ways)? How can we demonstrate osmosis (3 ways)? In terms of relationships between substances, how can we define “hypertonic”, “isotonic”, and “hypotonic”? What is the relationship between the size of a molecule and its rate of diffusion? ____________________________________________________________________________ Supplies ? Materials Needed Active Transport: Baker’s Yeast 0.75% Na2CO3 0.02% Neutral Red Erlenmeyer Flasks Flame or Hot Plate Brownian Movement: India Ink (Carmine Dye or Whole Milk may be substituted) Slides and Cover Slips Diffusion: Beaker of Distilled Water 1.5% Agar-agar (or Gelatin) in petri plate Potassium Permanganate (KMnO4) Crystals Potassium Dichromate (K2Cr2O7) Crystals Methylene Blue Crystals Metric Ruler (to measure in mm) Osmosis—Thistle Tube: Thistle Tube Osmometer Salt, Sugar Distilled Water Scale Artificial Cell: Dialysis Tubing or Sacs Sugar String Beakers Distilled Water Scale Osmosis and Red Blood Cells: Distilled Water; Salt Solutions: 12-15% Salt Salt Solutions: 0.85% Salt (This is human, isotonic saline.) Blood (Human or Animal): Lancets and Alcohol if Human Blood is used Slides and Cover Slips Microscope ____________________________________________________________________________ Key Terms ? Active Transport Brownian Movement Cell Physiology Dialysis Diffusion Gradient Hypertonic Hypotonic Isotonic Membrane Transport Osmosis Passive Transport ____________________________________________________________________________ Introduction ? The individual cell is a dynamic microcosm, demonstrating in miniature all