experiment membrane transport- departmentof zoology, u b c(实验膜运输- departmentof动物学、u b c).doc
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Experiment
Membrane Transport
Objectives ? Referring to energy, what two ways can substances enter a cell?
What is active transport?
What is passive transport?
How is osmosis related to diffusion?
How can we demonstrate active transport?
How can we demonstrate Brownian movement?
How can we demonstrate diffusion (2 ways)?
How can we demonstrate osmosis (3 ways)?
In terms of relationships between substances, how can we define “hypertonic”, “isotonic”, and “hypotonic”?
What is the relationship between the size of a molecule and its rate of diffusion?
____________________________________________________________________________
Supplies ? Materials Needed
Active Transport:
Baker’s Yeast
0.75% Na2CO3
0.02% Neutral Red
Erlenmeyer Flasks
Flame or Hot Plate
Brownian Movement:
India Ink (Carmine Dye or
Whole Milk may be
substituted)
Slides and Cover Slips
Diffusion:
Beaker of Distilled Water
1.5% Agar-agar (or Gelatin)
in petri plate
Potassium Permanganate
(KMnO4) Crystals
Potassium Dichromate
(K2Cr2O7) Crystals
Methylene Blue Crystals
Metric Ruler (to measure
in mm)
Osmosis—Thistle Tube:
Thistle
Tube Osmometer
Salt, Sugar
Distilled Water
Scale
Artificial Cell:
Dialysis Tubing or Sacs
Sugar
String
Beakers Distilled Water
Scale
Osmosis and Red Blood Cells:
Distilled Water;
Salt Solutions: 12-15% Salt
Salt Solutions: 0.85% Salt
(This is human, isotonic
saline.)
Blood (Human or Animal):
Lancets and Alcohol if
Human Blood is used
Slides and Cover Slips
Microscope
____________________________________________________________________________
Key Terms ? Active Transport
Brownian Movement
Cell Physiology
Dialysis
Diffusion
Gradient
Hypertonic
Hypotonic
Isotonic
Membrane Transport
Osmosis
Passive Transport
____________________________________________________________________________
Introduction ? The individual cell is a dynamic microcosm, demonstrating in miniature all
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