[EMSA中文操作说明.docxVIP

Procedure for Electrophoretic Mobility Shift Assay (EMSA) This kit has been optimized for use with polyacrylamide mini (8 × 8 × 0.1cm) gels. For larger gels, adjust electrophoresis conditions and detection reagent volumes accordingly. A. Plan Binding Reactions ? Understanding the Control EBNA System Include a complete set of three reactions each time an EMSA is performed. These reactions and expected results for the Control Epstein-Barr nuclear antigen (EBNA) System, which is included with the kit, are described in Table 1.Table 1. Description of control reactions and expected results.Reactio