strategiesfor cryo- electron tomography（strategiesfor electron tomography cryo-）.docVIP

Strategies for Cryo Tilt-Series Acquisition The strategy for tilt-series acquisition for cryo-ET has several major differences compared to plastic section ET. 1) Plastic-embedded specimens can tolerate much higher dose without significant damage. A plastic tilt-series under “normal” conditions can get up to a total dose of 10,000 e-/A2; where in cryo we strive for a total dose of 100 e-/A2 or less (total dose means all ancillary imaging like eucentricity plus the tilt-series dose). In cryo we therefore use the “low-dose mode”, where Focus and Trial/Tracking images are displaced along the t