b i t c1311 introductionto biotechnology（b我t c1311 introductionto生物技术）（137页）.docVIP

BIOL 1414 Introduction to Biotechnology Course Equivalent to BITC 1411 Laboratory Manual Ninth Edition Fall 2009 Linnea Fletcher, Evelyn Goss, Patricia Phelps, and Angela Wheeler ISBN: BIOL1414009 Table of Contents Introduction 3 Safety in the ACC Laboratory 7 Math Skills for the Laboratory 13 Documentation and the Lab Notebook 21 Basic Tools in the Biotechnology Laboratory 24 Using a Micropipetter 29 Calibrating Lab Instruments 33 Molar Solutions and Dilutions 38 RNA Isolation 45 Transformation of E. coli 52 Plasmid Isolation 56 Restriction Enzyme Mapping of DNA 59 Green Fluorescent Protein Purification 66 Protein Electrophoresis of GFP Samples 70 DNA Fingerprinting by Alu PCR 76 Bioremediation: Environmental Clean-Up 80 DNA Fingerprinting by Southern Blot 84 ELISA for HIV 90 Bioinformatics 95 Appendix A: ACC Lab Safety Procedures 116 Appendix B: Hints for Solving Numerical Problems 122 Appendix C: Summary of Chemical Hazards, MSDS, Chemical Labels and Solution Prep Forms 124 Appendix D: Graphing Data 130 Appendix E: Summary of Good Laboratory Practices 133 Appendix F: Agarose Gel Electrophoresis with Ethidium Bromide 136 Introduction Welcome to your first course in biotechnology! This course will emphasize its laboratory component to reflect the importance of your training in biotechnology skills. Keep in mind as you work your way through this manual the specific purposes in each exercise. They will prepare you for your first job in a biotechnology laboratory, so keep a careful record of your experience. If you carefully document and archive your work, this information will be easy for you to access later and your experiences will be more valuable in your later work. To help you to develop an archiving system for