【2017年整理】实验性短暂脑缺血发作磷脂水平的变化及养血清脑颗粒的作用.docVIP

实验性短暂脑缺血发作磷脂水平的变化及养血清脑颗粒的作用 牛建平1 薛萍2 伍期专1* 李丹1 姚存姗1 王燕华1 包鹤丘1 摘要：目的 研究短暂性脑缺血发作(TIA)对磷脂的影响以及中成药养血清脑颗粒的作用。 方法 尾静脉注射过氧化物并窒息建立小鼠TIA模型,灌胃喂食不同剂量养血清脑,诱发4次后隔日断头取脑测定血浆和脑组织中溶血磷脂酸(LPA)以及酸性、中性磷脂等的含量。结果 不给药组的评分为：0.969 ± 0.790， 给予2g/kg体重养血清脑颗粒， 评分为：0.500 ± 0.408（P0.05）。TIA小鼠血浆LPA比对照明显升高, 分别为：6.12 ± 0.35和2.11 ± 1.27。治疗可逆转这一变化。为：4.88 ± 1.55。TIA小鼠脑组织酸性磷脂与对照相比明显降低。分别为：2.27 ± 1.13 及6.58 ± 2.77μmole/g 治疗组小鼠可上升至4.26 ± 1.99μmole/g。结论 实验性TIA小鼠出现血浆和脑组织中LPA及其它磷脂水平异常。养血清脑有逆转作用，可能用于缺血性脑卒中的治疗。 关键词 养血清脑颗粒 短暂性脑缺血发作 磷脂 Changes of Phospholipids in Experimental Transient Ischemic Attack and the Effect of YangXuiQingNau (Jianping Niu, Ping Xue, Qizhuan Wu，Dan Li, Zunshan Yao, Yanhua Wang, Heqiou Bao) Abstract: Objective To study the effectiveness and mechanism of YangXuiQingNau in treatment for TIA. Methods Transient Ischemic Attack (TIA) was induced by injection ____________________________ 作者单位1．北京大学第一医院神经内科100034 2．铁道部北京铁路总医院干部医疗科 * 通信作者100034 北京西什库大街8号 北大医院神经生化研究室 of peroxide in mice. Different doses of YangXuiQingNau were feed every day. Lysophosphatidic acid , neutral and acidic phospholipid of both brain and plasma were assayed. Results The stroke score of mice feed with YangXuiQingNau(2g/kg body weight) was 0.500 ± 0.408, while the score of control was 0.969 ± 0.790 (p0.05). Plasma LPA and neutral phospholipid of mice with TIA were increased compared with health controls. These were 6.12 ± 0.35 and 2.11 ± 1.27. Administration of YangXuiQingNau decreased it to 4.88 ± 1.55. The acidic phospholipid of brain tissue decrease in TIA mice. YangXuiQingNau also partly reverse the decrement. These were 2.27 ± 1.13 , 6.58 ± 2.77μmole/g and 4.26 ± 1.99μmole/g respectively. Conclusion Phospholipid level changes in mice with experimental TIA. YangXuiQingNau has beneficial effect on them. It may be used in patients with TIA. 近年来的研究表明：磷脂(溶血磷脂等)异常在心脑卒中的发生中起着重要的作用[1，2]。但是，目前对于短暂性脑缺血发作磷脂水平变化的实验研究还比较缺乏。而且临床上一些有TIA样症状的病人，治疗后症状不易恢复，溶血磷脂水平也降低很慢。 中成药养血清脑颗粒有