WholecelliosensorforPCBanalysisasedonopticaldetection.docVIP

Whole cell biosensor for PCB analysis based on optical detection P Gavlasova1,3,*, G Kuncova1, L Kochánkova2 and M Mackova3 1Institute of Chemical Process Fundamentals, Czech Academy of Sciences, Rozvojová 135, Prague 6, 165 02, Czech Republic 2Department of Environmental Chemistry, Faculty of Environmental Technology, ICT, Technická 5, Prague 6 , 166 28, Czech Republic 3Department of Biochemistry and Microbiology, Faculty of Food and Biochemical Technology, ICT, Technická 5, Prague 6 , 166 28, Czech Republic *author for correspondence: email: gavlasova@icpf.cas.cz Abstract. Whole cell optical sensor of polychlorinated biphenyls (PCBs) based on production of coloured intermediates by silica entrapped cells is described. The reusable sensor (WCB) was prepared by co-immobilization of cells Pseudomonas species 2 with biphenyl in the silica matrix. The sensors were exposed to 13 individual PCB congeners, the commercial PCB mixture Delor 103, PAHs (anthracene, pyrene, phenanthrene), solvents (toluene, xylene), naphthalene, 1-methylnaphthalene and dibenzofuran. The results show that silica entrapped cells produce yellow intermediates (absorbance λmax = 398 nm) selectively in the presence of two PCB congeners: 2,4,4′-trichlorobiphenyl (2,4,4′-CB) and 2,4′,5-trichlorobiphenyl (2,4′,5-CB) and Delor 103. The PCBs detection was not influenced by PAHs. Naphthalenes and solvents decreased production of yellow intermediates. In the presence of dibenzorufane, PCBs detection was interfered with development of orange metabolites. The WCB′s detection limit was 1.2 ± 0.4 mg.L-1 (Delor103) and 0.2?±?0.08?mg.L-1 (2,4,4′CB), response time 3 hours, reproducibility ± 5%, reusability 3 times and ≥ 4 weeks storage stability was demonstrated. Introduction Polychlorinated biphenyls are highly persistent toxic compounds; which accumulate in the food chain and can negatively affect the health of different organisms. In Czechoslovakia PCB mixtures, called Delor, had been produced. Despite, Del