X射线诱导建立皮质发育障碍大鼠模型的病理学研究.docVIP

X射线诱导建立皮质发育障碍大鼠模型的病理学研究 冯占辉1 ,马勋泰2 ,于云莉1,楚兰1 , 蔡登华1 ,黄 华3 , 晏 勇3*(550004贵州，贵阳医学院附属医院神经内科1； 646000四川,泸州医学院附属医院神经内科2；400016重庆，重庆医科大学附属第一医院神经内科,重庆市神经病学重点实验室3) [摘要] 目的：探讨建立皮质发育障碍(disorder of cortical developments，DCDs)模型的最佳剂量方法：妊娠17的SD大鼠同时设立对照组。：统计各组仔鼠的存活率观察仔鼠。采用Morris水迷宫实验测试各组仔鼠学习和空间记忆能力。大形态、常规病理检查用红藻氨酸诱导各组仔鼠癫痫发作，比较其痫性发作阈值和死亡率。结果：结论：可以制作出理想的仔鼠DCDs模型(1Department of Neurology, Affiliated Hospital of Guiyang medical college, Guizhou 550004；2Department of Neurology, Affiliated Hospital of Luzhou medical college, Sichuan Province，646000； 3Department of Neurology, First Affiliated Hospital of Chongqing medical university, Chongqing ，400016, China) [Abstract] Objective：To investigate the optimal dose of the established animal models with disorders of cortical development (DCDs) induced by x-ray. Methods：Pregnant Sprague-Dawley rats were given x-ray with different radiation doses at embryonic day 17roup A 125cGy, Group B 150cGy, Group C 175cGy, Group D 200cGy, Group E 225cGy) .The following aspects were observed in all groups. ①The survival rate of rat offspring in each group were calculated. ②Morris-water-maze experiment were adopted to test the learning and memory capacity of rat offsprings in each group on P28d. ③We examined the changes of brain pathology in cerebral cortex and hippocampus to confirm the types of DCDs on P56d.④ Kainic acid were used to induce seizure and compared their seizure threshold and mortality induced by seizure on P63d. Results：Morris-water-maze experiment showed that the escape latency and times of crossing platform of rat offsprings in the group C and the group D were always longer than those of the control group(P0.05). 3) The incidence rate of DCDs of offsprings in the group C and group D reached 100%. 4)The latent period of Kainic acid inducing seizure was shortened in the group B and group C(P0.05), but the time of epileptic state and the mortality induced by seizure of rat offspring in the second group were the same as that of control gr