细胞工程翻工译.docVIP

第22段： In 2007, Li et al. [94] proposed their microfluidic device for single cell analysis. The chip was fabricated by standard 1-photomask with low cost method. The device consists four reservoirs, four channels and one open region which contain a cell retention chamber. Reservoir one was used for cell introduction and washing, whereas reservoir two was used for reagent delivery. Reservoir three and four were used as waste reservoirs. By using this chip, they quantified dynamic Ca2+ mobilization of a single cardiomyocyte during its spontaneous contraction. Also, they monitored successfully dynamic responses from various external stimulation such as daunorubicin (cardiotoxic chemotherapeutic drug), caffeine, and isoliquiritigenin (herbal anticancer). Their results also prove that anticancer drugs have less effect on the Ca2+ of the cardiomyocytes. This device has quantified the cellular response of single cardiomyocytes, discovery of heart diseases drug and cardiotoxicity testing. 翻译：在2007年。李某提出了单细胞分析的微流控装置。芯片是依据标准1-photomask制造的，并且成本低。装置四个水库，四通道和一个开放区域Ca2+的数量，及在其中是自发的收缩他们成功地监测的动态响应各种外部刺激如柔红霉素（心脏毒性的化疗药物），咖啡因，和异甘草素（抗癌他们的研究结果也明抗癌药物对Ca 2+影响较小。这个单个心肌细胞的细胞反应,发现心脏疾病的药物和毒性测试。In 2010, Mellors et al. [95] proposed an electrophoretic and electrospray ionization based microfluidic device for single cell analysis. The device was fabricated on corning borosilicate glass substrate by using standard photolithography and wet chemical etching technique. Figure 12 shows the schematic of the microfluidic device, where A was a cell loading reservoir and B was buffer loading, which intersects with the separation channel 翻译：在2010年，Mellors等提出了基于单细胞分析微流控装置一个电泳和电喷雾电离。康宁硼硅玻璃使用标准光刻和湿化学蚀技术。显示了微流装置的示意图,细胞加载水库缓冲加载,与分离相交的通道。This intersection zone was a cell lysis zone. CS was an electro-osmotic pump which was connected with an electrophoretic separation channel and electrospray orifice. Cells can flow throughhydrodynamically or electrically to the intersection zone, where cells were electrically