芪丹颗粒剂治疗大鼠实验性矽肺的形态学观察..docVIP

芪丹颗粒剂治疗大鼠实验性矽肺的形态学观察 辛洪涛，张华楠，张维东 【摘要】 目的 探讨芪丹颗粒剂抗矽肺纤维化作用。方法 60只Wistar大鼠随机分为六个组：正常对照组、模型组、芪丹1组、芪丹2组、汉甲1组、汉甲2组。除正常对照组外，其余各组采用气管注射染尘（50mg/鼠）方法建立动物模型，芪丹1组和汉甲1组分别从造模后第2天灌注芪丹颗粒剂（3125mg/kg）和汉防己甲素( Tetrandrine ,Tet)（22mg/kg）；芪丹2组和汉甲2组分别从造模30天后灌注芪丹颗粒剂和汉防己甲素（用量同前），5个月后处死。测定各组大鼠肺脏器系数及羟脯氨酸含量，同时对肺组织作HE染色、VG染色和网状纤维染色（Gomorri），普通光镜下观察各组矽结节的细胞构成，胶原纤维及网状纤维改变；同时取肾组织作HE染色，普通光镜下观察肾脏病理改变。结果 芪丹组、汉甲组的肺系数、羟脯氨酸含量均低于模型组，差异有显著性（P＜0.05）。模型组主要以Ⅲ～Ⅳ级矽结节为主，胶原纤维粗大、排列致密，网织纤维稀疏，芪丹颗粒及汉防己甲素治疗组主要以Ⅱ级矽结节为主，胶原纤维细小、排列疏松，网织纤维密集；汉甲组大鼠出现肾损伤，其余各组大鼠肾组织正常。结论 芪丹颗粒剂有延缓和减轻矽肺纤维化的作用，与汉防己甲素比较无明显差异，但无肾毒性。 【关键词】 芪丹颗粒剂；矽肺鼠；肺脏器系数；羟脯氨酸含量；病理学观察 Effects of Qidan granule on Pathological Observation of Experimental Silicosis 【Abstract】 Objective To studay the anti-fibrotic effects of Qidan granule in rats. Methods The rats were randomly divided into six experimental groups: normal group, model group, Qidan group one, Qidan group two, Tetrandrine group one and tetrandrine group two. All the rats except normal group were treated with silicon dioxide (50mg/rat) by intratracheal instillation to induce silicosis. Qidan group and Tetrandrine group were treated with Qidan granule(3125mg/kg)or treated with Tetrandrine (22mg/kg)respectively . All the rats were sacrified after 5 months.Lung/body coefficient and were measured. Paraffinembedded lung sections with HE staining、VG staining and Gomorri staining was observed under light microscop. Results In Qidan group and Tetrandrine group ,the Lung/body coefficient and content of Hydroxyproline is lower compared with model group(P0.05). Model group was mainly Ⅲ～Ⅳ grade silicotic nodule,which contained thick collagen and sparse reticulum fibe; Qidan group and Tetrandrine group were mainly Ⅱgrade silicotic nodule, which contained tiny collagen and intensive reticulum fibe. Tetrandrine group showed up injury of kidney, others were normal. Conclusion Qidan granule extract inhibited and reduced remarkably silicotic fibrosis in rats. 【Key Words】 Qian granule, Experimental Silicosis, L