04光学显_微镜.pptVIP

Example of Aperture Stop evil stray ray (prolongs graduation) aperture stop (pinhole) Aperture Stops The aperture stop (AS) determines the amount of light reaching the image Note the AS increases the f/# AS needed to “clean up” optical signals such as: in front of a detector, such as in  confocal microscopy in front of an objective to reduce stray light Focusing Light in the Real World (1) Things to consider when focusing: #1 Light can not be focused through an aperture smaller than a diffraction-limited spot for a standard lens @ 630 nm, this is ~ 3 ?m diffraction thru circular ap