03哺乳动_物组织基因组DNA提取.pptVIP

2005-3-22 2005-3-21 2002级生物技术专业 2004-2005（下）分子生物学实验 Genomic DNA Extraction from Mammal Tissue 哺乳动物组织基因组DNA提取 一、实验目的(Experimental Purpose) After the experiment is finished, students should be able to extract genomic DNA from mammal tissue, and to run an agarose gel. 真核生物染色体DNA组装不同层次的结构 We can add the eathylene diamine tetraacetic acid (EDTA) to keep the integrity of DNA. EDTA can chelate Mg2+ and reduce deoxyribonuclease (DNase) activity, because these enzymes require Mg2+ to work. 为了进一步保护DNA样品的完整性，通常需要在缓冲液中添加乙二胺四乙酸(EDTA)以整合Mg2+，这样将会减少脱氧核糖核酸酶(DNase)的活性，因为该酶必须在有Mg2+存在时才会起作用。