jc_Cel_Micromanipulation_and_Nanoscale_Probing.pptVIP

Cell Micro-Manipulation and Nanoscale Probing Techniques Different Experimental Techniques and Mechanical Models for Cells Method used to observe will dictate response Experimental observations lead to different models Linking mechanical signals to biological signals How cells mechanically respond under physical loads Different Experimental Techniques and Mechanical Models for Cells Optical TrappingA Hands Off Manipulation of Cells Optical TrappingA Hands Off Manipulation of Cells Optical TrappingA Hands Off Manipulation of Cells Experimental Setup Objective used to converge laser External lens on 3D mount Leveled beam splitters and blocking filters Atomic Force MicroscopyMultiple Levels of Information Multiple applications for cellular probing Classical, Imaging live and fixed cells for a total 3D representation of adherent cellular structure Cellular Elastography, Measuring the local viscoelastic properties of live cells Molecular Force Probe, Measuring the dissociation kinetics of surface receptors through single molecule manipulation Atomic Force MicroscopyMultiple Levels of Information AFM Basic Setup and principles Surface mediated cantilever deflection Photodetector measuring reflected laser displacement Measure surface deformation with 10pm resolution Atomic Force MicroscopyMultiple Levels of Information AFM Cantilevers tips are equipped with a specific geometry for function Atomic Force MicroscopyMultiple Levels of Information Viscoelastic studies using AFM probing techniques Quantifying local cellular elastic properties with regard to the cytoskeleton Molecular Force ProbeAFM without the Microscopy Functionalizing Cantilevers as a live biological substrate Receptor binding in native environment observed Theoretical models are used to extract unstressed kinetic values Bell’s Model Quantum DotsAs Applied to Biological Problems Unique optical and spectroscopic properties Broad adsorption Narrow/Tunable emission Photobleaching resistance Long Lumin