04_赤霉素课稿.pptVIP

* 第四章 赤霉素 生物合成 微生物中的合成途径 植物中的合成途径 在微生物中的合成途径 Gibberellin (GA)-biosynthetic pathways in Fusarium fujikuroi, indicating reactions of the main (3β-hydroxylated) and the minor (non-hydroxylated) pathways as well as side reactions catalysed by the enzyme P450-1. All reactions affected in the cpr-Gf mutant and dependent on cytochrome P450 oxidoreductase (CPR) are marked. Dotted arrows Proposed sequence of reactions. GGPP Geranylgeranyl diphosphate, CPPent-copalyl diphosphate Tudzynski， Appl Microbiol Biotechnol (2005) 66: 597–611 Thomas and Sun， Plant Physiology, June 2004, Vol. 135, pp. 668–676, 在植物中的合成途径 比较在微生物与植物中合成途径的差异 Tudzynski，Appl Microbiol Biotechnol (2005) 66: 597–611 赤霉素的代谢途径 Olszewski, et al., The Plant Cell, S61–S80, Supplement 2002 Inactive catabolites 赤霉素合成缺失突变体 Phenotype of GA-deficient mutants in Arabidopsis and in barley. A, Five-week-old wild-type (WT) Arabidopsis and the GA-deficient ga1-3 mutant. B, Two-week-old WT Himalaya and a GA-deficient mutant grd2 (Chandler and Robertson, 1999). Thomas and Sun， Plant Physiology, June 2004, Vol. 135, pp. 668–676, WT ga1-3 WT grd2 GA突变体 Barley GA Mutants. Plants are of equal age and are (from left to right) Himalaya (wild type), grd2 (GA-deficient putative GA3ox mutant [Chandler andRobertson, 1999]), Sln1d (dominant dwarf), and sln1c (slender). Olszewski, The Plant Cell, S61–S80, Supplement 2002 sln1c sln1d grd2 WT 施用GA会促进植物伸长 Fig. 1. Three-week-old pumpkin seedlings, treated with GA4 and/or the GA-biosynthesis inhibitor LAB150978 (Jung et al. 1986). Treatment from left to right: control, GA4 (10)6 M), LAB150978 (10)6 M), and GA4 plus LAB150978 (both 10)6 M) Lange， Planta (1998) 204: 409±419 control 加GA4 加GA4合成抑制剂 同时加GA4和GA4合成抑制剂 赤霉素信号转导 Thomas and Sun, Plant Physiology, June 2004, Vol. 135, pp. 668–676, Gomi and Matsuoka Current Opinion in Plant Biology 2003, 6:489–493 In the absence of GA, DELLA protein (SLR1/RGA) directly or indirectly inhibitsthe expression of GA-induced genes, including GAMYB. KGM inh