(杜仲、茯苓配伍对小鼠免疫功能的影响.docVIP

杜仲、茯苓配伍对小鼠免疫功能的影响 黑龙江大学 陈喜君 王艺 【摘要】 目的：观察杜仲、茯苓联合作用对小鼠免疫功能的影响。方法：40只小鼠随机分4组，分别为正常对照组（I）、杜仲组（II）、茯苓组（III）及复方组（IV）。灌胃给药后，测定外周血T细胞的表达情况及脾抗体生成数。结果：经统计学分析，与对照组相比，试验组组均能显著升高CD4+及CD4+/CD8+的比值（P＜0.05），但II组对抗体生成数无明显作用。结论：杜仲对机体的细胞免疫有显著的增强作用，对体液免疫无明显作用。茯苓可显著提高抗体生成量，但CD4+及CD4+/CD8+比值低于杜仲组。茯苓和杜仲联合使用，在体液免疫和细胞免疫两方面增强机体的免疫力，具有增效作用。 【关键词】 杜仲，茯苓，配伍，免疫力 The effect of compatibility Eucommia ulmoides with Poria cocos on immunological function in mice Abstract Objective: To observe the immunity effect of Eucommia ulmoides, Poria cocos and their compatibility. Method: The 40 mice were randomly divided into 4 groups: normal control group (I), Eucommia ulmoides group (II), Poria cocos group (III) and compatibility group (IV). Detecting the quantity of T lymphocyte in peripheral blood and the antibody-producing cells in spleen after the drug was given intragastrically. Results: Through statistical analysis, treatment group could increase CD4+ and CD4+/CD8+ ratio compared with the control group (P＜0.05). But II was no significant effect on the antibody-producing cells. Conclusion: Eucommia ulmoides had a significant effect in the enhancement of cellular immunity and no obvious effect on humoral immunity. Poria cocos can significantly increase the amount of the antibody cells, but CD4+ and CD4+4/CD8+ ratio lower than II group. Compatibility of Eucommia ulmoides with Poria cocos has a synergistic effect to enhance the bodys immune system in both humoral and cellular immune. Key Words Eucommia ulmoides, Poria cocos, Compatibility；Immunity 杜仲和茯苓均为我国传统中药，已有2000多年的药用历史。杜仲在中药古典《神农本草经》、《本草纲目》中就被列为药之上品[1]。近年来，国内外许多学者对杜仲的化学成分和药理作用进行了大量研究，发现杜仲具有调节血压、降低血糖、抑制肿瘤细胞生长等药理作用。《中国药典》规定其树皮作为中药杜仲入药[2]。茯苓为多孔菌科卧孔菌属真菌茯苓的干燥菌核，多依附松树根生长[3]。该菌是著名而且用途极广的传统中药材，又是著名的食用菌。1 材料与方法 ’s、荧光素标记的抗小鼠抗体 PE-CD8+、FITC-CD4+等。 1.3 分组与给药 将40只小鼠，随机均匀分为空白对照组、杜仲单方组、茯苓单方组和杜仲茯苓复方组4组。 表1试验动物分组Ficoll-Hy-paque淋巴细胞分离液获得外周血单个核细胞，用PBS液洗涤后调整细胞浓度为（1.0~1.5）×109/L。在1mL单个核细胞溶液中加入FITC-CD4+单抗和PE-CD8+单抗1μL，置4℃冰箱避光孵育1h后用冷PBS液洗涤，10g/L多聚甲醛固定后，用FACSCalibu