黑曲霉诱导子促进岩黄连悬浮细胞中生物碱的合成.docVIP

黑曲霉诱导子促进岩黄连悬浮细胞中生物碱的合成 作者：程华 张玉芹 余龙江 敖明章 【摘要】 目的为了提高岩黄连悬浮培养细胞中生物碱的产量。方法以不同浓度黑曲霉诱导子处理岩黄连悬浮培养细胞。结果生物碱的产量随着诱导时间的延长迅速提高，到第6天达到最大值；添加50 mg/L诱导子时生物碱产量最高，达到89.2 mg/L，比对照组高出2.5倍，当诱导子浓度进一步升高时，生物碱产量反而降低；随着诱导子浓度从0 mg/L增大到150 mg/L，细胞生物量反而逐渐减少。结论 黑曲霉诱导子可促进岩黄连细胞中生物碱的合成，最佳诱导子浓度是50 mg/L；过高浓度的诱导子并不利于细胞生长和生物碱合成，细胞生物量的减少是导致生物碱产量下降的一个重要原因。该处理提供了一种简单有效的方法提高岩黄连细胞培养中生物碱的产量。 【关键词】 黑曲霉 悬浮细胞培养 岩黄连 诱导子 　　Abstract：ObjectiveTo improve the production of alkaloids in Corydalis saxicola Bunting suspension cell cultures.MethodsElicitor prepared from Aspergillus niger was used to treat the suspension cells on day 12.ResultsThe results showed that the production of alkaloids increased with the extension of time after treatment and the greatest value of alkaloid production was obtained on day 6. Cultures treated with 50 mg/L elicitor generated the highest alkaloid production, 89.2 mg/L, which was about 2.5-fold of that in the control.Alkaloid production significantly decreased in response to increased elicitor concentration from 50 mg/L to 150 mg/L. When elicitor concentration increased from 0 to 150 mg/L, biomass of cultures inversely decreased.ConclusionThis elicitor is effective to enhance alkaloid biosynthesis in cell suspension cultures of C. saxicola and the optimal concentration for alkaloid biosynthesis is 50 mg/L. Higher concentration of the elicitor is unfavorable to not only cell growth but also alkaloid production. This decrease of biomass induced by higher concentration of elicitor is a main reason for the suppression of alkaloid production. This treatment provides a simple and effective approach to enhance alkaloid biosynthesis of C. saxicola cells. 　　Key words：Aspergillus niger; Cell suspension cultures; Corydalis saxicola Bunting; Elicitor 　　真菌诱导子被广泛用于促进植物细胞中代谢产物的合成，在多种植物的悬浮培养细胞中显示出高效诱导性，成为近年来体外细胞培养生产有用天然产物的研究热点［1～3］。黑曲霉（Aspergillus niger）是一种广泛分布在土壤和空气中的真菌，由黑曲霉制备的诱导子被证明能促进多种植物细胞培养物中特定代谢产物的合成，Huang等［4］使用来源于黑曲霉的诱导子，提高了红豆杉细胞培养中抗癌药物—紫杉醇的含