红茶菌调节小鼠免疫功能的实验研究.docVIP

红茶菌调节小鼠免疫功能的实验研究 作者：李波清，孟玮，王志强，于敏，孙旭红 【关键词】 红茶菌;,,小鼠;,,免疫功能 　　摘要：目的探讨红茶菌对小鼠免疫功能的调节作用。方法Balb/c小鼠30只，体重18～22 g，随机分为两组，用红茶菌液给小鼠灌胃30 d后处死，以MTT法测定NK细胞的杀伤活性；以腹腔巨噬细胞吞噬白色念珠菌的方法评价巨噬细胞吞噬功能；以胸腺细胞增殖法和脾细胞增殖法分别测定IL1和IL2的生物活性。结果红茶菌能显著提高NK细胞的杀伤活性；巨噬细胞吞噬功能明显增强；红茶菌灌胃组IL1和IL2活性分别为：0.549±0.024，0.405±0.018；对照组分别为：0.341±0.021，0.361±0.011，实验组与对照组比较，IL1，IL2活性得到显著提高(P＜0.01)。结论 红茶菌能显著增强小鼠的免疫功能。 　　关键词：红茶菌; 小鼠; 免疫功能 　　Abstract：ObjectiveTo investigate the regulative effect of black tea fungus on the immunological function of mice. MethodsThirty Balb/c mice(weight 18～22 g) were randomly divided into two groups. All the mice were sacrificed after they had been given black tea fungus in intragastric administration for thirty days. The killing activity of NK cells were detected by MTT method, and peritoneal macrophage swallowing C. albicans test was used to appraise the phagocytes function. The activities of interleukin 1 and interleukin 2 were separately detected by mouse thymocyte or splenic cell proliferation test.ResultsThe activities of IL-1 and IL-2 of mice were 0.549?0.024, 0.405?.018 in experimental group respectively; they were 0.341?0.021 and 0.361?.011 respectively in control group. They were significantly higher in experimental group than those in control group (P＜0.01). The killing activity of NK cell and the phagocytes function of the macrophages in vitro were also markedly promoted(P＜0.01).ConclusionThe black tea fungus can enhance the immunological function of mice significantly. 　　Key words：Black tea fungus; Mice; Immunological function 　　红茶菌又称“海宝”或“胃宝”，是在我国民间广为流行的一种保健饮品，为我国传统医药的重要组成部分。前期研究中我们发现红茶菌对小鼠具有显著的抑瘤作用，为进一步探讨其抑瘤机制，我们通过用红茶菌对小鼠进行胃内灌注的方法，观察红茶菌对小鼠免疫功能的调节作用。 　　1 材料与方法 　　1.1 动物Balb/c小鼠，体重18～22 g，雌雄兼有，引自北京大学基础医学部实验动物研究中心。 　　1.2 细胞株K562细胞由北京大学基础医学部免疫系提供。 　　1.3 试剂红茶菌，本室保存菌种，总菌数为5.5×107CFU/ml，RPMI1640（Gibco公司），LPS（Sigma公司），白色念珠菌（本室保存），ConA（北京医药站），MTT（Sigma公司），DMSO（国产）。 　　1.4 仪器全自动酶标仪（Multiskan MS），CO2培养箱（Forma公司），低速冷冻离心机（IEC公司），电子精