黄芪多糖对2型糖尿病大鼠骨骼肌组织glut4表达的影响.docVIP

黄芪多糖对2型糖尿病大鼠骨骼肌组织GLUT4表达的影响 【摘要】 目的探讨研究黄芪多糖(APS)对2型糖尿病大鼠的作用及对骨骼肌组织GLUT4表达的影响。方法SD大鼠随机分为:正常对照组、黄芪多糖对照组(APS组)、2型糖尿病组(TIIDM组)和2型糖尿病黄芪多糖治疗组(TIIDM +APS组)，5周后测血糖、血清胰岛素和GLUT4的水平。结果TIIDM组和TIIDM +APS组血糖均高于对照组（Plt;0.01），TIIDM+APS组血糖低于TIIDM组（Plt;0.01）；各组间血胰岛素水平差别无显著性（Pgt;0.05）；TIIDM组骨骼肌组织GLUT4的表达低于对照组和TIIDM +APS组（Plt;0.01）。结论APS可降低TIIDM大鼠血糖水平，其机制与提高糖尿病大鼠骨骼肌中GLUT4表达有关。 【关键词】 黄芪多糖 2型糖尿病 葡萄糖转运蛋白4 　　Abstract:ObjectiveTo investigate the effect of Astragalus polysoccharide(APS)on expression of GLUT4 in the skeletal muscle tissue of TIIDM rats.MethodsSD rats were randomly divided into four groups:normal control group, normal control plus astragalus polysoccharide group(APS group)，TIIDM model group(TII DM group)，diabetic model plus astragalus polysoccharide therapy group(TII DM+APS group).The expression of GLUT4 were detected.ResultsThe levels of blood glucose of rats in TIIDM group and TII DM+APS group were significantly higher than those in control group and control+APS group (Plt;0.01),and the level of blood glucose in TIIDM+APS group was significantly lower than that in TIIDM group (Plt;0.01),levels of insulin were not significantly different among these groups（Pgt;0.05）.Expressions of GLUT4 in the skeletal muscle tissues of rats in TIIDM group was significantly lower than those in both control group，control+APS group and TIIDM+APS group.ConclusionAPS can decrease the levels of blood glucose in type II diabetic rats,and its mechanisms might be closely related to the elevation of the level of GLUT4 in the skeletal muscle tissues of rats. 　　Key words:Astragalus polysoccharide; Type II diabetes mellitus; Glucose Transporter 4 黄芪多糖（Astragalus polysaccharide，APS）是从豆科植物黄芪中提取出来的有较强生物活性的大分子复合物。初步研究表明，APS具有良好的降糖效果，且毒副作用小，但其降糖作用机制目前尚未完全阐明［1］。本研究运用APS治疗高脂饮食喂养加小剂量链脲佐菌素（STZ）注射诱导2型糖尿病（Type II Diabetes Mellitus，TIIDM），旨在通过进一步观察APS降糖效果及其对骨骼肌组织葡萄糖转运蛋白4（Glucose Transporter 4, GLUT4）表达的影响，为其临床应用提供实验依据。 　　1 材料与方法 　　1.1 材料健康清洁级雄性SD大鼠40只，体重180～200 g，购自武汉