黄芩素治疗子宫内膜异位症的作用研究.docVIP

黄芩素治疗子宫内膜异位症的作用研究 【摘要】 目的观察黄芩素对大鼠子宫内膜异位症防治作用及其机制。方法采用手术移植法制备大鼠子宫内膜异位症模型,大鼠随机分为正常对照组、模型对照组和黄芩素高、低剂量组，测定大鼠的血清TNFα，IL6，IL8含量及脾脏NK细胞活性，采用免疫组织化学的方法检测异位内膜标本中Bcl2，ICAM1的表达。结果黄芩素治疗组与模型组相比血清TNFα，IL6，IL8活性明显降低，脾脏NK细胞活性显著增高，异位内膜标本中ICAM1， Bcl2表达明显下降。结论 黄芩素对子宫内膜异位症大鼠具有治疗作用，其作用机制可能与其抑制TNFα，IL6，IL8产生，抑制ICAM1 ，BcI2的表达以及增强脾脏NK细胞活性有关。 【关键词】 黄芩素；子宫内膜异位症；自然杀伤细胞；细胞间黏附分子1 　　The Therapeutic Effects of Scutellarin on Endometriosis in Rats 　　Abstract：ObjectiveTo investigate the therapeutic effects and mechanism of scutellarin on endometriosis in rats.MethodsThe endometriosis model of rats was created by surgical implantation and rats were randomly divided into four groups. At the end of the experiment,the activities of serum TNFα，IL6，IL8 were observed, the expression of Bcl2 and ICAM-1 were also observed by immunohistochemistry and the activity of nature kill cell in spleen was measured. ResultsThe activities of serum TNFα，IL6，IL8 in scutellarin groups were lower than those in the model control group , the expressions of Bcl2，ICAM1 in scutellarin groups were lower than those in the model control group ,the activity of nature kill cell in spleen of scutellarin groups rose more significantly than that of the model control group .ConclusionScutellarin may have the therapeutic effect on the endometriosis of rats by inhibiting the producing of serum TNFα，IL6，IL8 , decreasing the expressions of Bcl2，ICAM-1 and enhancing the activity of nature kill cell in spleen. 　　Key words：Scutellarin; Endometriosis; NK cell; ICAM1 子宫内膜异位症(endometriosis EM) 是一种常见的妇科疾病，主要指子宫内膜在子宫腔被覆面以外的地方种植生长，是一种具有侵袭性的良性病变。该病的临床表现主要为顽固性的痛经，腹痛及不孕等，成为危害当今育龄妇女健康及生活质量的一种很重要的疾病。EM在育龄妇女中发病率约15%左右，在不孕妇女中高达50%，其发生机制可能与体腔上皮化生学、激素、免疫、环境、遗传学说等因素相关［1，2］。黄酮类化合物是一类低分子的广泛分布于植物界的天然植物成分,具有多方面的生物活性,如:抗炎、抗病毒、抗氧化、抗衰老、免疫调节以及抗肿瘤作用等。黄芩素（scutellarin）是从黄芩中提取的一种黄酮类单体化合物［3］。本实验通过建立大鼠子宫内膜异位症模型后给予黄芩素进行治疗，观察黄芩素对子宫内膜异位症大鼠细胞因子，ICAM1及凋亡相关因素的影响，为探讨子宫内膜异位症的治疗提供理论和实验依据。 　　1 材料与方法 　　1