黄芩提取物抗炎镇痛耐缺氧及抗疲劳作用的研究.docVIP

黄芩提取物抗炎镇痛耐缺氧及抗疲劳作用的研究 作者：熊娟 欧阳昌汉 黄胜堂 【摘要】 目的探讨黄芩苷对局灶性脑缺血再灌注损伤的保护作用及其机制。方法线栓法制备大鼠大脑中动脉局灶性脑栓塞模型，缺血2 h，再灌注24 h。评价神经功能状态，测定脑梗塞体积和丙二醛（MDA）和一氧化氮（NO）含量、超氧化物歧化酶（SOD）和髓过氧化物酶（MPO）活性以及血脑屏障通透性。称重法测定组织脑水肿含量。结果黄芩苷能显著降低缺血再灌注后脑梗塞体积，改善神经功能状态，降低脑组织MDA、NO含量、血脑屏障通透性和脑水肿程度，升高SOD活性并降低MPO 活性，与模型组比较，具有显著性差异（Plt;0.05或Plt;0.01）。 结论 黄芩苷对大鼠脑缺血再灌注损伤具有明显的保护作用，其机理与其清除氧自由基、抗炎和减轻脑水肿有关。 【关键词】 黄芩苷 脑缺血 再灌注损伤 脑水肿 　　Abstract:ObjectiveTo investigate the protective effect of baicalin on cerebral injury induced by transient focal ischemia and its mechanisms. MethodsTransient focal cerebral ischemia injury model in rats was induced by occlusion of the right middle cerebral artery for 2 h, followed by 24 h reperfusion. The infarction volume and neurological deficit determined by the methods of TTC staining and the Longas score were used to evaluate the effect of baicalin on cerebral injury. The levels of malondialdehyde (MDA), nitric oxide (NO), activities of superoxide dismutase (SOD) and myeloperoxidase (MPO) and blood-brain barrier (BBB) permeability in brain were measured by spectrophotometer. The content of brain water was assayed by weighing method.ResultsPretreatment with baicalin markedly reduced brain infarction volume and neurological deficit induced by transient ischemic insult, inhibited MPO activity, decreased NO, MDA and brain water content, and BBB permeability, increased SOD activity.ConclusionBaicalin might play a protective effect on cerebral ischemia reperfusion injury through inhibiting inflammatory process, peroxidation and decreasing brain water contents. 　　Key words:Baicalin; Cerebral ischemia; Reperfusion injury; Brain water content 　　黄芩Scutellaria baicalensis Georgi.是一种主要生长在我国北部的传统中药材，具有清热燥湿、泻火解毒、止血、安胎等作用，其主要有效成分为黄酮类，包括黄芩苷（baicalin）、黄芩素、汉黄芩苷和汉黄芩素，其中以黄芩苷的含量最高。近年研究表明，黄芩苷具有抗炎［1］、抗氧化活性［2］，有理由推测它对缺血性损伤疾病具有保护作用。本室前期的研究已证实黄芩苷对心肌缺血再灌注损伤具有较好的保护作用［3］。本文探讨了局灶性脑缺血再灌注时黄芩苷对脑梗塞体积和脑水肿的影响，观察其对缺血性脑损伤是否也具有保护作用。同时，为了探讨黄芩苷的神经保护机制，我们探讨黄芩苷对血脑屏障通透性以及炎性细胞浸润