经鼻黏膜给予mbp68.docVIP

经鼻黏膜给予MBP68 作者：孙博, 杨硕, 彭海生, 乔慧, 曹京燕, 金连弘, 李呼伦 【关键词】 鼻黏膜耐受;,MBP68 　　　　; AIM: To explore the synergistic effect of MBP6886 and 8799, on the inhibition of experimental autoimmune encephalomyelitis (EAE) in Lewis rat by nasal administration. METHODS: Three different MBP peptides(MBP6886, 8799, and the nonencephalitogenic peptide 110128) were synthesized and administrated nasally to Lewis rat on day11, 10, 9, 8 and 7 prior to immunization with the guinea pig MBP (gpMBP) + CFA, which was used to induce EAE. The protective effect on Lewis rat from EAE by the MBP peptides was evaluated. RESULTS: Protection was achieved with the encephalitogenic peptides MBP6886 and 8799, MBP6886 being more potent, but not with MBP110128. Neither MBP6886 nor 8799 used alone conferred complete protection to gpMBPinduced EAE. In contrast, nasal administration of a mixture of MBP6886 and 8799 completely blocked gpMBPinduced EAE even at lower dosage than being used alone. Rats tolerized with MBP6886+8799 nasally showed decreased T cell responses to MBP, reflected by lymphocyte proliferation and IFNγ ELISPOT assays. Rats tolerized with MBP6886+8799 also had abrogated MBPreactive IFNγ and TNFα mRNA expression in lymph node cells compared to rats receiving MBP110128 nasally, while similar low levels of MBPreactive TGFβ and IL4 mRNA expressing cells were observed in the two groups. CONCLUSION: Nasal administration of encephalitogenic MBP peptides can induce antigenspecific T cell tolerance and confer incomplete protection to gpMBPinduced EAE, and MBP 6886 and 8799 have synergistic effecs. Nonregulatory mechanisms are proposed to be responsible for tolerance development after nasal peptide administration. 　　; 目的: 探讨鼻黏膜给予MBP6886和8799协同免疫预防Lewis大鼠实验性自身免疫性脑脊髓炎（EAE）的作用。方法: 合成3条不同的碱性髓鞘蛋白（MBP）多肽（MBP6886、 8799和非致脑炎性肽段110128）, 在用豚鼠MBP（gpMBP）加弗氏完全佐剂免疫Lewis大鼠前的11、 10、 9、 8和7 d, 经鼻黏膜分别给予MBP多肽, 观察其对EAE的保护作用。结果: 致脑炎性肽段MBP6886和8799都有保护作用, 其中MBP6886的保护作用更强; 而MBP110128没