食管癌mgmt、survivin蛋白的表达及其临床意义.docVIP

食管癌MGMT、survivin蛋白的表达及其临床意义 【摘要】 目的 研究食管癌MGMT、survivin蛋白的表达及其临床意义。方法 应用免疫组化（EnVision二步法）检测56例食管鳞状细胞癌、20例腺样结构食管癌和9例小细胞癌组织中MGMT、survivin蛋白的表达，并分析其与临床病理诸因素之间的关系。结果 小细胞癌与鳞状细胞癌及腺样结构食管癌的MGMT蛋白表达比较均有显著性差异（Plt;0.01）；低分化鳞癌MGMT蛋白阳性表达率为45.45%，低于高、中分化鳞癌的阳性率（84.44%，Plt;0.05）。食管鳞状细胞癌survivin阳性表达率明显高于腺样结构食管癌（Plt;0.05）。结论 MGMT、survivin蛋白表达与食管癌的组织学类型有密切关系，可作为食管癌预后判断的参考指标。 【关键词】 食管癌；MGMT；survivin；免疫组化 　　Expression and Clinical Significance of MGMT and survivin in Esophageal Carcinoma 　　Abstract：Objective To investigate the expression and clinical significance of MGMT and survivin in esophageal carcinoma.Methods Expression and their relationship with clinicopathological parameters of MGMT gene and survivin proteins was detected by immunohistochemical techniques(EnVision) in 56 cases of esophageal squamous cell carcinoma,20 cases of carcinoma with adenoid structure and 9 cases of small cell carcinoma.Results Significant difference was observed in the expression of MGMT between small cell carcinoma and esophageal squamous cell carcinoma、esophageal carcinoma with adenoid structure（Plt;0.01）; The positive rate of Survivin expression in poor differentiated esophageal squamous cell carcinoma(45.45%) was lower than that in well and moderate differentiated esophageal squamous cell carcinoma(84.44%，Plt;0.05).The expression of survivin was significantly higher in esophageal squamous cell carcinoma than in esophageal carcinoma with adenoid structure (Plt;0.05).Conclusion The expression of MGMT and survivin was signicantly correlated with histological type of the esophageal carcinoma,which may be used as important indexes to evaluate the prognosis of esophageal carcinoma. 　　Key words：Esophageal neoplasms；MGMT；survivin；Immunohistochemistry 　　0 引言 6氧甲基鸟嘌呤DNA甲基转移酶(Ｏ6methylguanine DNA methytransferase,ＭＧＭＴ)是一种高效的DNA直接修复酶,对保护细胞免受烷化剂损害,防止细胞癌变和死亡具有极为重要的作用。survivin是凋亡抑制蛋白家族的新成员,在凋亡抑制和细胞周期中发挥作用。目前,国内外文献中对MGMT、survivin基因在食管癌中的表达报道甚少，两者在腺样结构食管癌组织的表达尚未见报道。