BiomaterialChemistryMethodsofModificationandImmobil.pptVIP

Powder is fed into the plasma flame most commonly via an external powder port mounted near the anode nozzle exit. The melted or partially melted particles impact the surface and rapidly solidify. The powder is so rapidly heated and accelerated that spray distances can be in the order of 25 to 150 mm. Plasma Spray * Adsorption of dithiobis(N-succinimidyl propionate) (DTSP) on a Au-electrode Fig. 3 Adsorption of dithiobis(N-succinimidyl propionate) (DTSP) on a Au-electrode surface and application of the self-assembled monolayer containing active ester groups for covalent coupling of HRP.? (E. Katz, J. Electroanal. Chem., 1990, 291, 257) 丙酸鹽 Horseradish Peroxidase 山葵過氧化氫酶 Applications Ag+ (aq) Ag (s) CD RP215 RP215 Biotin Streptavidin Gold Nanoparticle Silver Particles EDC/NHS Coupling Affinity Coupling EDC/NHS Coupling * * * * * * * * * * * * Biomaterial Chemistry: Methods of Modification and Immobilization Reporter: Lily Ou Instructor: Hsien-Chang Chang Nov. 30, 2011 * Surface Functionalization Layer Coating Surface Activation (1) (2) Blocking Immobilizing Motivations Therapeutic uses Drug delivery Medical uses Artificial organs Tissue engineering Diagnostic uses * Ratner, B. D.; Hoffman, A. S.; Schoen, F. J.; Lemons, J. E. Biomaterials Science. 2nd ed. San Diego: Elsevier Academic Press, 2004. Surface Modification Activation Want to modify materials in such a way that only the outermost surface is modified. Functionalize materials for further steps while retaining the physical properties of the surface. What materials? Polymers, metals, ceramics, and glasses What biomolecules? Enzymes, antibodies, and affinity proteins * Ratner, B. D.; Hoffman, A. S.; Schoen, F. J.; Lemons, J. E. Biomaterials Science. 2nd ed. San Diego: Elsevier Academic Press, 2004. Can be divided into 3 categories: (1) Physical alteration - Electrostatic - Affinity (2) Chemical alteration - EDC/NHS Coupling - Self-Assembled Monolayer (SAM) (3) Physicochemical alteration - Plasma