第五章-核磁共振方法解析蛋白质结析.pptVIP

核磁共振方法解析蛋白质结构 核磁共振是解析蛋白质结构的一种重要方法 优点：不需结晶；可研究动力学。 缺点：受分子量限制，需要标记。 Malate synthase G (82kDa) Richard Robert Ernst Ernst was awarded the Nobel Prize in Chemistry in 1991 for his contributions towards the development of Fourier Transform nuclear magnetic resonance spectroscopy and the subsequent development of multi-dimensional NMR techniques. Kurt Wüthrich He was awarded half of the Nobel Prize in Chemistry in 2002 for his development of nuclear magnetic resonance spectroscopy for determining the three-dimensional structure of biological macromolecules in solution. 核磁共振的原理 自旋量子数（I）