光谱法研究苯甲酸钠与DNA的体外作用…………………张智渊414.docVIP

光谱法研究苯甲酸钠与DNA的体外作用 Research on in vitro Interaction between DNA and Benzoic Acid by Spectroscopic Spectra 化学与分子工程学院98级 张 智 渊 摘 要 采用紫外、荧光光谱的分析方法，研究了苯甲酸钠与小牛胸腺DNA的体外相互作用。实验结果没有发现苯甲酸钠和DNA之间存在能够明显影响两者各自的紫外光谱或荧光发射光谱性质的相互作用。表明在体外苯甲酸钠未对DNA的构象有明显的影响，也未对DNA中各种碱基有明显作用。 关键词：DNA 苯甲酸钠 紫外光谱 荧光光谱 Abstract Research on the in vitro interaction between DNA and benzoic acid by spectroscopic spectra was carried out. The DNA solution and the benzoic acid solution of different concentration were mixed (pH=7.40, PBS buffer), then the interaction between DNA and benzoic acid was detected by the fluorescen