东乐膏指纹图谱和中药及其土壤中有机氯农药残留量的测定.docVIP

河北大学 硕士学位论文 东乐膏指纹图谱研究和中药及其土壤中有机氯农药残留量的测 定 姓名：韩媛媛 申请学位级别：硕士 专业：分析化学 指导教师：阎正  摘 要 ? 摘?要? ? 本文共分三章 第一章 文献综述概括了中药指纹图谱的定义和属性 总结了中药指纹图 探讨了研究中药指 谱研究的意义和作用 纹图谱的技术问题 详细介绍了这一领域国内外研究现状 引用文献 101篇 第二章 本研究以东乐膏为载体 初步研究了中药橡胶膏剂指纹图谱的分析 方法 采用? ??相结合的方式对其中不同性质的成分进行研究 可以较为全 面地反映东乐膏中的化学组成 ? 利用 法对中药橡胶膏剂东乐膏中挥发性成分进行了分析 建立了东乐膏 确定?个色谱峰作为特征指纹峰 并对东乐膏中的主要挥发性成分 分析周期为?? 线性范围为? 利用??法对中药橡胶膏剂东乐膏进行了分离 色谱条件 色谱柱 ?指纹图谱 冰片的含量进行了快速分离测定 线性相关系数为??? 分析 建立??指纹图谱 ? ? ?? 甲醇??醋酸体积百分比浓度 为流动相 流速 ? ??? 检测波长 ? ? 该方法简便 第三章 准确 应用 重现性好 法对白芍 样品以石油醚?丙酮???在索氏提取器中提取 提取液以 符合相关技术要求 ? 白芷及其所种植的土壤中有机氯农药残留的相 关性进行了初步研究 浓硫酸净化 样品采用???弹性石英毛细管柱分离 实用 应用此法考察了土壤中农药残留量对相关种植中草药的影响 检测 该方法简便 稳 定 ???? 关键词中药指纹图谱 东乐膏 GC HPLC有机氯农药  ABSTRACT ABSTRACT This paper consists of three chapters. In CHAPTER ONE, literatures and reviews which contains definitions and attributions of fingerprint of Chinese medicine and the importance and effects of researching are summarized. Research progress in China and abroad are introduced in detailed. The technological problems of research of fingerprint of Chinese medicine are discussed. 101 pieces of literatures are cited. In CHAPTER TWO, Donglegao is the carrier of this study. Analytical method of the fingerprint of the traditional Chinese rubber electuary medicine is studied primarily. Integration method of GC and HPLC is employed in the study of different character contents and chemical components in Donglegao can be reflected overall. The fingerprint of Donglegao which is a traditional Chinese rubber electuary medicine has been studied and provides the quality information by capillary gas chromatography. The samples were pretreated by means of ultrasonic wave extraction with alcohol. The capillary column was DB-WAX ( 30m 0.25mm 0.25 m JW Scientific). The content of borneolum in Donglegao was quickly determined. The analytical period was only 6min. The linear ran