和分子杂交.ppt

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2017-02-03 发布于天津
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和分子杂交.ppt

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和分子杂交

分子杂交点印迹（dot-blot）法 dot and slot hybridization allele-specific oligonucleotide, ASO Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. ASO杂交 His Leu Thr Pro Glu Glu Lys Ser Ala CAT CTG ACT CCT GAG GAG AAG TCT GCC His Leu Thr Pro Val Glu Lys Ser Ala CAT CTG ACT CCT GTG GAG AAG TCT GCC CTG ACT CCT GAG GAG AAG TCT CTG ACT CCT GTG GAG AAG TCT Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. CTG ACT CCT GAG GAG AAG TCT CTG ACT CCT GTG GAG AAG TCT ASO杂交 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. Southern印迹（Southern blot）分子杂交点印迹（dot-blot）法 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 医学遗传学基因操作 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 本章节重点重组DNA技术分子杂交技术 PCR技术及应用 Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 重组DNA技术（recombination DNA technique）指将目的DNA片段或人工合成的基因，通过载体在体外重组、转移并插入到另一种生物的基因组中，使其表达为新的性状或用于进一步的研究，是基因操作的核心技术。包括DNA克隆（DNA cloning）和分子杂交（molecular hybridization）。重组DNA Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 构建转化扩增（克隆）分离和检测重组DNA Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 重组DNA技术流程简图 ligase vector restriction endonuclease foreign DNA bacterial cell host Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. 限制性内切核酸酶限制酶：能够识别DNA链的特定部位并能进行切割。 TypeⅠ TypeⅡ TypeⅢ ? Evaluation only. Created with Aspose.Slides for .NET 3.5 Client Profile 5.2.0.0. Copyright 2004-2011 Aspose Pty Ltd. EcoRⅠ 属名种名菌株名酶的序号限制性内切核酸酶限制酶：能够识别DNA链的特定部位并能进行