淫羊藿苷在不同骨质疏松大鼠模型中肠道吸收代谢研究.docVIP

淫羊藿苷在不同骨质疏松大鼠模型中肠道吸收代谢研究 　　[摘要] 该实验比较淫羊藿苷在不同骨质疏松大鼠模型中肠道吸收代谢情况。分别采用卵巢摘除和环磷酰胺灌胃的方法复制2种大鼠骨质疏松的模型，在此基础上，对其进行淫羊藿苷的在体肠灌流，HPLC检测并计算淫羊藿苷的表观渗透系数及代谢产物的生成量；Western blot考察2种模型肠段LPH酶的表达情况。实验结果表明，卵巢摘除和环磷酰胺4.5 mg?kg-1灌胃可诱发大鼠骨密度降低，成功复制骨质疏松模型；20 μmol?L-1的淫羊藿苷灌流液在假手术组大鼠各肠段的表观渗透系数Papp按十二指肠、空肠、回肠、结肠顺序依次为5.695，5.224，1.492，0.520；在卵巢摘除组中为3.876，3.608，0.863，0.291；在生理盐水组中为4.945，3.601，1.990，1.042；在环磷酰胺致骨质疏松模型组为3.301，2.108，1.209，1.233；此外，蛋白表达结果显示2种模型组肠段中LPH酶的表达水平均比正常组低。淫羊藿苷在2种骨质疏松模型中的吸收代谢比假手术组和生理盐水组低，不同骨质疏松模型大鼠肠段的LPH酶表达均降低，是导致淫羊藿苷肠道吸收代谢降低的原因之一。 　　[关键词] 淫羊藿苷；骨质疏松；LPH酶；吸收代谢；肠灌流 　　[Abstract] To compare the intestinal absorption and metabolism of icariin in different osteoporosis rat models. Ovariectomy and intragastric administration of cyclophosphamide were used to establish two kinds of rat osteoporosis models. Then the rat intestinal perfusion was conducted， and HPLC was used to measure and calculate the permeability coefficients of icariin in different intestines and production amount of metabolites. Western blot was used to detect LPH enzyme expression in two models. Experimental results showed that both ovariectomy and intragastric administration of cyclophosphamide 4.5 mg?kg-1 could reduce rat bone density and successfully construct the rat osteoporosis models. The apparent permeability coefficient Papp of 20 μmol icariin in duodenum， jejunum， ileum， colon was 5.695， 5.224， 1.492， 0.520 respectively in sham operation group；3.876， 3.608， 0.863， and 0.291 in ovariectomized group；4.945， 3.601， 1.990， 1.042 in normal saline group；3.301， 2.108， 1.209， 1.233 in cyclophosphamide-induced osteoporosis model group. In addition， the protein expression levels of LPH enzyme in two model groups were lower than those in normal group. The absorption and metabolism of icariin in two kinds of osteoporosis models was lower than that in sham operation group and normal saline group；the reduction of expression level of LPH enzymes in rat intestine of different osteoporosis models was one of the reasons f