武大分子生物学内部课件.pptVIP

DNA strands can separate and reassociate DNA STRUCTURE (3) Key terms to understand Denaturation (变性) Hybridization (杂交) Annealing/renature (复性) Absorbance (吸收度) Hyperchromicity (增色性) Tm (melting point) (熔点) DNA TOPOLOGY DNA TOPOLOGY (1) Structure (1): Linking number is an invariant topological property of covalently closed, circular DNA (cccDNA) Linking number is the number of times one strand have to be passed through the other strand in order for the two strands to be entirely separated from each other. Species of cccDNA Plasmid and circular bacterial chromosomes Linear DNA molec