Promocell原代细胞培养概要.ppt

How can I calculate the number of population doublings? The number of doublings (n) can be calculated using the following formula: n = (log N - log N0) / log 2 N: total viable cells at harvestN0: total viable cells at seedlog 2 = 0.301 * * * * Which problems in cell culture have you experienced? What do you think is the most often occuring problem in cell culture? Hyphae (Amphoterizin B or Nystatin) Occuring extracellularly ? That‘s why they are typically detected in the media/supernatant of cell cultures Enzymimmunoassay: immunoassay based on polyclonal antibodies against the four princi