中学生物学专业英语.pptVIP

Molecular Cytogenetic Characterization of a New Wheat-Thinopyrum intermedium Partial Amphiploid Resistant to Powdery Mildew and Stripe Rust Name: jiao xiaoyan Major:Biologicaltechnology Catalogue The content The problem need to be solve The material of the study The method of the study The result of the study The problem which havent solved The content A partial amphiploid, TE253, derived from crosses between the cultivar Yannong 15 and Thinopyrum intermedium (Host) Barkworth D.R. Dewey was characterized by cytogenetic observations,disease resistance tests and genomic in situ hybridization (GISH). Mitotic observations showed that most plants of TE253 had 56 chromosomesnome demonstrated that TE253 consisted of 2 St-genome chromosomes, 8 J S-ge-nome chromosomes, 2 SAT chromosomes and 2 J-St trans-located chromosomes. . The problem need to be solve to determine the genomic composition of a partial amphiploid TE253 by GISH probed with St genomic DNA from Ps. strigosa and to evaluate its resistance to both powdery mildew and stripe rust as well. The material of the study Plant Materials The plant materials used in this study included Th. intermedium, Ps. strigosa , the partial amphiploid TE253, and the common wheat varieties Yannong 15Chinese Spring (CS), Mingxian 169 and Huixianhong. The method of the study 1.Cytogenetic Analyses(细胞遗传学分析） To observe the number andphology of chromosomes in Mitotic metaphase and meiotic metaphase I cells . 2.Reaction to powdery mildew and stripe rust 用不同的品种接种两种病，观察感病情况。 3. GISH Purpose：detect the alien chromosome in TE253 Prober: St-genomic DNA Blocker: CS-DNA 。 1.Cytogenetic Analyses(细胞遗传学分析） Operation process: 1.Seeds were germinated at 25°C → maintained at 4°C for 24 h→ transferred to 25 °C→Roots 1–2 cm were cut and treated in ice water for 24 h → fixing in Carnoy’s solution →stain and squash the root tips in carbol fuchsin→ observe mitotic chromo