哈佛分子生物学讲义核酸特性结构和功能培训教案.pptVIP

The “Linker” Histone H1/H5 Largest histone (21 kD), less conserved Binds 165 bp: “Chromatosome” Facilitates chromatin compaction 19 Are Nucleosomes “Positioned?” 1. Sequence at Unique Position 2. Micrococcal Nuclease Digest 3. Restriction Digest 4. Southern Blot Discrete Band Positioning Smear Random Orientation 17 Implications of Translational Positioning 18 N-ARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTE- H3 N-SGRGKGGKGLGKGGAKRHRKVLRDNIQ - H4 N-SGRGKQGGKTRAKSKTRSSRAGL- H2A N-SDPAKSAPAAKKGSKKAVTKTQKKDGKKRRKSRKES- H2B H3 -ERA-C H4 -RTLYGFGG-C H2A -ELNKL