p21p73及PTEN在头颈部多原发癌中的表达及意义.doc

p21、p73及PTEN在头颈部多原发癌中的表达及意义 作者：黄方 作者单位：福建医科大学附属第二医院耳鼻咽喉科， 福建 泉州 362000 【摘要】 目的 探讨p21、p73与PTEN在头颈部多原发癌组织中的表达与意义。方法 采用免疫组织化学方法对30例头颈部多原发癌患者的42份标本、42例头颈部单发癌及30例来源于头颈部的正常上皮组织标本中p21、p73与PTEN蛋白表达进行检测，并将检测结果作统计学分析。结果 p21、p73与PTEN蛋白在头颈部多原发癌组织中阳性表达率分别为69.05％、57.14％和71.43％；在单原发癌组阳性表达率分别为76.19％、40.48％和76.19％；在正常上皮组织中阳性表达率分别为96.66％、13.33％和100.00％。在多原发癌及单发癌组织中p21与PTEN蛋白失表达率以及p73蛋白过表达率均显著高于正常上皮组织（P＜0.05）；三个指标在多原发癌与单发癌组织中的表达差异均无统计学意义。结论 p21与PTEN蛋白失表达以及p73蛋白表达上调，参与头颈部多原发癌和单发癌的形成机制，但还不能认为其在头颈部多原发癌与单发癌形成中的作用有差别。 【关键词】 多原发癌 头颈部肿瘤 p21 p73 PTEN 免疫组织化学 Abstract: Objective To investigate the effects of p21, p73 and PTEN expressions in multiple primary cancers of the head and neck. Method Expressions of p21, p73 and PTEN were determined by the SP immunohistochemical technique in 42 samples of multiple primary cancer tissues, 42 cases of marched single cancer tissues of the head and neck, and 30 cases of normal epithelial tissues of the head and neck. Results Positive expression rates of p21, p73 and PTEN in multiple primary cancer tissues and single cancer tissues of the head and neck were 69.05％, 57.14％, 71.43％ and 76.19％, 40.48％, 76.19％, respectively, and those in normal epithelial tissues were 96.66％, 13.33％ and 100.00％. The loss expression rates of p21 and PTEN and the overexpression rates of p73 in multiple primary cancer tissues and single cancer tissues of the head and neck were all significantly higher than those in normal epithelial tissues(P＜0.05), but no significant differences were found between multiple primary cancer tissues and single cancer tissues. Conclusion The loss of expression of p21 and PTEN and the upregulated expression of p73 may play important roles in tumor genesis of multiple primary cancer and single cancer of the head and neck, but no differences have been found till now between multiple primary cancer and single cancer. Key words: Multiple primary cancers; Head and neck neoplasms; p21; p73; PTEN; Immun