PeproTech公司ELISA操作指南视频(视屏地址字幕及翻译).doc

学习啦-PeproTech ELISA操作指南视频已上传!为能够更好的熟悉和掌握PeproTech的ELISA试剂盒及其技术，PeproTech专门制作了ELISA操作指南视频，供各位老师和同学观摩！视频网址为：/v_show/id_XMzQ3Mzk3NTg4.html 或 /programs/view/tzU8m93o0tI/，欢迎大家的批评和指正！该视频为英文讲解，为便于大家理解，请看下面的英文原文及翻译：The following video tutorial described how to run a sandwich ELISA, also known as enzyme-linked immunosorbent assay, using a general PeproTech protocol.接下来的实验指导讲述PeproTech夹心法ELISA，即酶联免疫吸附试验的通用步骤The capture, standard, and detection are supplied as stable lyophilized products, and should be stored at -20℃ until ready for use.捕获抗体、标准品和检测抗体均为冻干粉，使用前应保存于-20Reconstituted Capture, Standard, and Detection components are only guaranteed to be stable for up to 2 weeks when stored at 4 ℃.捕获抗体、标准品和检测抗体重悬后，在4时最长保存2周。If you have reconstituted the EDK, and plan on using it for a duration greater than two weeks, aliquot and store at -20 for up to 6 months.如果您已重悬了EDK的各组份，并准备在2周之后使用，请将重悬的组分分装并冻存于-20，最长可保存6个月。In contrast to the other three EDK components, the Avidin-HRP vial comes ready to use与EDK的其它三个组分不同，Avidin-HRP为即用型In order to avoid harmful repeated freeze/thaw cycles, or long-term storage at 4 which mean advisory functionality. Aliquot the Avidin-HRP into ten 6uL vials upon receipt and stored at -20 ℃ are stable for up 2 years from the date of receipt.目的是避免反复冻融或长期4保存对该组分的功能损害。收到Avidin-HRP后，立即将其分装为6ul/管，共10管，冻存于-20，最长可保存2年Phase 1:第1阶段：Coating the plate with capture antibody捕获抗体包板Centrifuge the vial briefly to bring the capture antibody to the bottom将捕获抗体稍作离心，使抗体集中于管底Reconstitute the capture body in sterile water to the concentration specified on the datasheet. And allow the vial to be reconstituted for minimum 10 minutes.用无菌水将捕获抗体重悬至说明书上要求的浓度，静置10分钟以使抗体完全溶解Centrifuge the reconstituted vial for 3 minutes at maximum speed重悬的抗体以最高速度离心3分钟Dilute the capture antibody in 1×PBS to the concentration specified on the datasheet.用1xPBS稀释捕获抗体至说明书上要求的浓度Gently mix or vortex the vial, try to ensure the air bubbles don’t mix into the solution,轻轻颠倒或振荡混匀