CRISPR-Cas9技术的发展与应用.docxVIP

CRISPR/Cas9技术的发展与应用Zujia.W摘要： 成簇规律间隔短回文重复序列/成簇规律间隔短回文重复序列关联蛋白(Clustered regularly interspaced short palindromic repeats/CRISPR-associated proteins, CRISPR/Cas) 技术是近两年出现的一种定点基因组编辑新工具，具有灵活、高效、廉价且易于操作等优点。这种新的基因编辑工具的出现，为基因功能、疾病分子机制的研究带来了便利,并且在研究和使用过程中不断创新发展，扩大了其应用范围。关键词：成簇规律间隔短回文重复序列/成簇规律间隔短回文重复序列关联蛋白 基因编辑 转录调控 基因治疗 ABSTRACT: Clustered regularly interspaced short palindromic repeats /CRISPR-associated proteins (CRISPR/Cas) has been developed as a new targeted genome editing tool since the last 2 years. Because its flexible, efficient, cheap and easy to operate, it quickly surpass the previous tec