ELISA KSU酶联免疫吸附su.pptVIP

An example of an ELISA experiment-Cont The sample is added to plate in duplicate or triplicate and then the mean result is calculated The quality control sample which is provided with the kit is treated as the test samples Results After reading the results the standard curve is drawn were the concentration is blotted on the X-axis and the absorbance on the Y-axis Concentration ng/ml Absorption nm Results-cont The standards concentrations is specified on the x-axis and the reading of each standard is specified on the y-axis and the standard curve is drawn Results-cont This standard curve is used to determine the unknown concentration of each sample by finding the opposite concentration to the absorbance Concentration ng/ml Absorption nm Results-cont The quality control sample concentration is determined from the standard curve and if the result is in the range given by the kit manufacturer the results could be accepted Useful sites /en/a543-direct-enzyme-linked-immunosorbent-assay-elisa ELISA /webcontent/animations/content/ELISA.html http://www.biology.ualberta.ca/facilities/multimedia/uploads/procedures/elisa-sound.swf ELISA Enzyme Linked Immunosorbent Assay Definitions Antibodies (also known as immunoglobulins abbreviated Ig) are gamma globulin proteins that are found in blood and are used by the immune system to identify and neutralize foreign objects, such as bacteria and viruses. Definitions- cont Antigens A substance that when introduced into the body stimulates the production of an antibody Immunoassay A laboratory technique that makes use of the binding between an antigen and its homologous antibody in order to identify and quantify the specific antigen or antibody in a sample Definitions- cont Analyte The sample being analyzed and in immunoasssays the analyte is either Antibody or Antigen Antigen Is present naturally in the body like hormones Is manufactured in special disease status for example human chorionic gonadotrophin hormone (H