肝脂素(YS2H)对小鼠S37肉瘤与B16黑色素瘤的抗肿瘤作用.doc

肝脂素（YS2H）对小鼠S37肉瘤 与B16黑色素瘤的抗肿瘤作用 乔艺、路建平▲、毛建群▲、沈祖铭★、林肇辉● 上海市第四人民医院耳鼻咽喉科 （上海 200081） 上海医科大学病理教研室 （上海 200032） 中国科学院上海药物研究所 （上海 200032） ●大连医科大学日中永和研究所 （大连 116027） 摘 要： 肝脂素（Heplipin，YS2H）是牛肝经放射线照射、发酵后经有机溶剂提取制备的脂溶性抗癌新药。本文用小鼠S37肉瘤和B16黑色素瘤荷瘤模型，监测肝脂素对这两株移植肿瘤的抗肿瘤作用。结果显示：不同剂量的肝脂素治疗对肿瘤生长均有抑制作用，其中YS2H对S37肉瘤和B16黑色素瘤的抑瘤率分别为68.4％和47.0％（3000mg/Kg）、57.2％和32.1％（1500mg/Kg）、47.5％和13.0％（1100mg/Kg），显示出明显的剂量效应关系，与生理盐水对照组相比有极其显著和非常显著性差异。其毒副作用小于环磷酰胺与榄香烯。S37瘤株用药组的肿瘤坏死面积（60.5±20.5％、75.5±21.4％）明显大于对照组（48.3±19.8％）。YS2H用药组肿瘤周边部单位面积内血管数明显少于溶剂对照组和CTX组。实验表明：肝脂素可抑制小鼠S37肉瘤和B16黑色素瘤的生长，可能成为临床有效的癌症治疗药物。其抗肿瘤机制可能与抑制肿瘤周边部新生血管的形成，导致肿瘤缺血坏死有关。 关键词：抗癌药，肝脂素，小鼠移植性肿瘤 本研究课题由日中永和协会及国家教育科研基金资助。 The Antitumor Efficacy of Heplipin (YS2H) On S37 Sarcoma and B16 Melanoma in Mice Qiao Yi、Jian-Ping Lu▲、Mao Jian-Qun▲、Shen Zu-Ming★、Hayashi K● Shanghai Fourth People’s Hospital (Shanghai 200081) Shanghai Medical University (Shanghai 200032） Shanghai Institute of Pharmacy, China Academy of Science （Shanghai 200032） JICA and Okayama University Medical School （Japan 700） Abstract Heplipin (YS2H) is a new anti-cancer remedy. It is made from irradiated and fermented cattle liver, abstracted through organic solvents. We used Kunming or C57 mouse implanted with S37 sarcoma or B16 melanoma as animal model to examine the efficacy of YS2H in vivo. Comparing with the Normal Saline (NS) fed control, YS2H administration reduced the tempo of tumor enlargement, tumor inhibit rates are 68.4% (3000mg/Kg/day, P0.001)、57.2% (1800mg/Kg/day, P0.01)、47.5% (1100mg/Kg/day, P0.05) in YS2H treated S37 sarcoma implanted mouse groups; and 47.0% (3000mg/Kg/day, P0.01)、32.1% (1800mg/Kg/day, P0.01)、13.0% (1100mg/Kg/day) in YS2H treated B16 melanoma host groups. In addition, histomorphologic examination showed that YS2H treated groups had more tumor necrosis (60.5?0.5% and 75.5?1.4%) than the NS treated controls (48.33?9.8%) in mice implanted with S37, (P0.05, P0.01). The blood vessel in 3000mg/Kg/day group was 1.39/field less than in control g