胰腺癌患者手术前后CD4CD8T细胞比例与IL4,IFNγ含量的关系.doc

胰腺癌患者手术前后CD4-CD8- T细胞比例与IL-4，IFN-γ含量的关系 作者：杜敏 作者单位：安徽医科大学附属省立医院 【摘要】 目的 研究胰腺癌患者手术前后外周血CD4-CD8- T细胞比例与IL-4、IFN-Γ含量变化及其相互关系。方法 分别应用流式细胞仪及ELISA法检测胰腺癌患者手术前后外周血中CD4-CD8- T细胞比例与IL-4、IFN-Γ含量。结果 20例胰腺癌患者术前CD4-CD8- T细胞占CD3+ T细胞的比例为(4.13%±1.81%)，术后CD4-CD8- T细胞占CD3+ T细胞的比例为(6.11%±1.40%)，两组之间差异有统计学意义(P0.01)。胰腺癌患者术前外周血中IL-4含量为（90.29±24.99）pg/ml，术后外周血中IL-4含量为（71.43±8.39）pg/ml，两组之间差异有统计学意义(P0.01)；术前外周血中IFN-Γ含量为（15.79±5.90）pg/ml，术后外周血中IFN-Γ含量为（20.08±6.60）pg/ml，两组之间差异有统计学意义(P0.01)。结论 胰腺癌患者手术前后CD4-CD8- T细胞比例变化可能影响IL-4、IFN-Γ含量的变化，胰腺癌患者手术前后测定CD4-CD8- T细胞比例、IL-4、IFN-Γ含量对判断机体的免疫水平具有一定的参考价值。 【关键词】 胰腺肿瘤 CD4-CD8- T细胞 白细胞介素4 干扰素Γ The relationship between CD4-CD8- T cells ratio of patients with pancreatic carcinoma before and after operation and IL-4，IFN-Γ levels DU Min， CHEN Jiong， LI Wenbo， et al. Department of General Surgery， Provincial Hospital Affiliated to the Anhui Medical University， Hefei 230001 Abstract Objective To investigate the relationship betweem CD4-CD8- T cells ratio in the peripheral blood of patients with pancreatic carcinoma before and after operation and IL-4， IFN-Γ levels. Methods Flowcytometer was used to analyze the CD4-CD8- T cells ratio in the peripheral blood of patients with pancreatic carcinoma before and after operation and detect the IL-4 and IFN-Γ levels with Elisa. Results The ratio of CD4-CD8- T cell in CD3+ T cell from 20 pancreatic carcinoma patients was (4.13%±1.81%) before operation; the ratio of CD4-CD8- T cell in CD3+ T cell from 20 pancreatic carcinoma patients was (6.11%±1.40%) after operation， significant deviation can be found between them （P0.01）. The IL-4 level in 20 pancreatic carcinoma patients was （90.29±24.99） pg/ml before operation; the IL-4 level in 20 pancreatic carcinoma patients was （71.43±8.39） pg/ml after operation， there was significant deviation between them （P0.01）. The IFN-Γ level in 20 pancreatic carcinoma patients was （15.79±5.90） pg/ml before operation; the IFN-Γ level